{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:39:53Z","timestamp":1773308393913,"version":"3.50.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Understanding how existing antivector immunity impacts live vaccine delivery systems is critical when the same vector system may be used to deliver different antigens. We addressed the impact of antivector immunity, elicited by immunization with attenuated<jats:italic>actA<\/jats:italic>-deficient<jats:italic>Listeria monocytogenes<\/jats:italic>, on the CD8<jats:sup>+<\/jats:sup>-T-cell response to a well-characterized lymphocytic choriomeningitis virus epitope, NP118-126, delivered by infection with recombinant<jats:italic>L. monocytogenes<\/jats:italic>. Challenges of immune mice with<jats:italic>actA<\/jats:italic>-deficient and with wild-type recombinant<jats:italic>L. monocytogenes<\/jats:italic>generated similar numbers of CD8<jats:sup>+<\/jats:sup>T cells specific for the NP118-126 epitope. High-dose immunization with<jats:italic>actA<\/jats:italic>-deficient<jats:italic>L. monocytogenes<\/jats:italic>resulted in substantial numbers of CD8<jats:sup>+<\/jats:sup>T cells specific for the<jats:italic>L. monocytogenes<\/jats:italic>LLO91-99 epitope in the effector and memory stages of the T-cell response. Challenge of these immune mice with recombinant<jats:italic>L. monocytogenes<\/jats:italic>resulted in rapid control of the infection and decreased CD8<jats:sup>+<\/jats:sup>-T-cell responses against both the secreted and nonsecreted form of the recombinant antigen compared to the response of nai\u0308ve mice. In contrast, mice immunized with a low dose of<jats:italic>actA<\/jats:italic>-deficient<jats:italic>L. monocytogenes<\/jats:italic>had \u223c10-fold fewer effector and memory T cells specific for LLO91-99 and a substantially higher CD8<jats:sup>+<\/jats:sup>-T-cell response against the recombinant antigen after challenge with recombinant<jats:italic>L. monocytogenes<\/jats:italic>. Although mice immunized with low-dose<jats:italic>actA<\/jats:italic>-deficient<jats:italic>L. monocytogenes<\/jats:italic>had a substantial recall response to LLO91-99, which reached the same levels by 5 to 7 days postchallenge as that in high-dose-immunized mice, they exhibited decreased ability to control<jats:italic>L. monocytogenes<\/jats:italic>replication. Thus, the level of antivector immunity impacts the control of infection and efficiency of priming responses against new antigens introduced with the same vector.<\/jats:p>","DOI":"10.1128\/iai.70.1.153-162.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:40Z","timestamp":1027764220000},"page":"153-162","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["CD8<sup>+<\/sup>-T-Cell Response to Secreted and Nonsecreted Antigens Delivered by Recombinant<i>Listeria monocytogenes<\/i>during Secondary Infection"],"prefix":"10.1128","volume":"70","author":[{"given":"Amy R.","family":"Tvinnereim","sequence":"first","affiliation":[{"name":"Department of Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara E.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Program in Immunology, The University of Iowa, Iowa City, Iowa 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John T.","family":"Harty","sequence":"additional","affiliation":[{"name":"Department of Microbiology"},{"name":"Interdisciplinary Graduate Program in Immunology, The University of Iowa, Iowa City, Iowa 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(91)90065-R"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5258.54"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.7.1359"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/32183"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6444"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00153-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5495.1354"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","volume":"139","year":"1987","unstructured":"Bishop, D. K., and D. J. Hinrichs. 1987. Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements. J. Immunol.139:2005\u20132009.","journal-title":"J. Immunol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.1.253-258.1999"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.2.657"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11890"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80540-3"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/4.12.1413"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.5.797"},{"key":"e_1_3_2_16_2","first-page":"107","volume":"3","year":"1995","unstructured":"Harty, J. T., and M. J. Bevan. 1995. Specific immunity to Listeria monocytogenes in the absence of IFN-\u03b3. Immunity3:107\u2013119.","journal-title":"Immunity"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830241223"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/369031a0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.001021"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11571"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.12.6833"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80470-7"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/353852a0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4049\/jimmunol.149.1.53","volume":"149","year":"1992","unstructured":"Schafer, R., D. A. Portnoy, S. A. Brassell, and Y. Paterson. 1992. Induction of a cellular immune response to a foreign antigen by a recombinant Listeria monocytogenes vaccine. J. Immunol149:53\u201359.","journal-title":"J. Immunol"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(99)01633-0"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80946-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.3987"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.1.208"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2196-2204.2000"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1303-1309.1999"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1997.tb01002.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"898","DOI":"10.4049\/jimmunol.160.2.898","volume":"160","year":"1998","unstructured":"White, D. W., and J. T. Harty. 1998. Perforin-deficient CD8(+) T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD95 and IFN-\u03b3 but requires TNF-\u03b1. J. Immun.160:898\u2013905.","journal-title":"J. Immun."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.4.613"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.70.1.153-162.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.70.1.153-162.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T15:34:22Z","timestamp":1682264062000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.70.1.153-162.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1128\/IAI.70.1.153-162.2002"],"URL":"https:\/\/doi.org\/10.1128\/iai.70.1.153-162.2002","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1]]},"assertion":[{"value":"2001-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-10-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}