{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:16:07Z","timestamp":1771654567874,"version":"3.50.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As a first step toward the design of an epitope vaccine to prevent contagious agalactia, the strongly immunogenic 55-kDa protein of\n            <jats:italic>Mycoplasma agalactiae<\/jats:italic>\n            was studied and found to correspond to the AvgC protein encoded by the\n            <jats:italic>avgC<\/jats:italic>\n            gene. The\n            <jats:italic>avg<\/jats:italic>\n            genes of\n            <jats:italic>M. agalactiae<\/jats:italic>\n            , which encode four variable surface lipoproteins, display a significant homology to the\n            <jats:italic>vsp<\/jats:italic>\n            (variable membrane surface lipoproteins) genes of the bovine pathogen\n            <jats:italic>Mycoplasma bovis<\/jats:italic>\n            at their promoter region as well as their N-terminus-encoding regions. Some members of the Vsp family are known to be involved in cytoadhesion to host cells. In order to localize immunogenic peptides in the AvgC antigen, the protein sequence was submitted to epitope prediction analysis, and five sets of overlapping peptides, corresponding to five selected regions, were synthesized by Spot synthesis. Reactive peptides were selected by immunobinding assay with sera from infected sheep. The three most immunogenic epitopes were shown to be surface exposed by immunoprecipitation assays, and one of these was specifically recognized by all tested sera. Our study indicates that selected epitopes of the AvgC lipoprotein may be used to develop a peptide-based vaccine which is effective against\n            <jats:italic>M. agalactiae<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.70.1.171-176.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:40Z","timestamp":1027764220000},"page":"171-176","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Mapping Antigenic Sites of an Immunodominant Surface Lipoprotein of\n            <i>Mycoplasma agalactiae<\/i>\n            , AvgC, with the Use of Synthetic Peptides"],"prefix":"10.1128","volume":"70","author":[{"given":"Antonella","family":"Santona","sequence":"first","affiliation":[{"name":"Porto Conte Ricerche, 07041 Alghero (SS)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Carta","sequence":"additional","affiliation":[{"name":"Porto Conte Ricerche, 07041 Alghero (SS)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peppinetta","family":"Fraghi\u0301","sequence":"additional","affiliation":[{"name":"Porto Conte Ricerche, 07041 Alghero (SS)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Turrini","sequence":"additional","affiliation":[{"name":"Dipartimento di Genetica, Sezione di Biochimica, University of Turin, 10126 Turin, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Current protocols in molecular biology 1987"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.151.3.1514-1522.1982"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.16.3.1062"},{"key":"e_1_3_2_5_2","first-page":"209","volume":"21","year":"1998","unstructured":"Buonavoglia, D., A. 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