{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:16:29Z","timestamp":1791915389069,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have developed a system in which a foreign antigen is delivered and expressed on the surface of an attenuated strain of\n            <jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>\n            YS-1 and have examined the ability of a such recombinant\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            strain to function as a mucosal vaccine vector. The C-terminal portion, including two repeat regions, R1 and R2, of the P97 adhesin of\n            <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>\n            strain E-1 was successfully translocated and expressed on the\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            YS-1 cell surface after it was fused to SpaA.1, a cell surface protective antigen of\n            <jats:italic>E. rhusiopathiae.<\/jats:italic>\n            BALB\/c mice subcutaneously immunized with the\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            recombinant strains developed specific antibodies against SpaA.1 protein and were protected from lethal challenge with the highly virulent homologous\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            Fujisawa-SmR strain, showing the efficacy of this heterologous-antigen expression system as a vaccine against\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            infection. To determine whether protective immune responses are induced in target species, newborn, specific-pathogen-free piglets were immunized intranasally with a recombinant strain designated YS-19. The immunized piglets developed specific anti-SpaA.1 immunoglobulin G (IgG) antibodies in their serum and were protected from death by erysipelas, showing that mucosal vaccination of piglets with YS-19 induces systemic immune responses. Furthermore, YS-19-immunized piglets showed higher levels of P97-specific IgA antibodies in the respiratory tract than did YS-1-immunized piglets. Thus,\n            <jats:italic>E. rhusiopathiae<\/jats:italic>\n            YS-1 appears to be a promising vaccine vector for mucosal delivery that can induce local and systemic immune responses.\n          <\/jats:p>","DOI":"10.1128\/iai.70.1.226-232.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:18Z","timestamp":1027763898000},"page":"226-232","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["<i>Erysipelothrix rhusiopathiae<\/i>\n            YS-1 as a Live Vaccine Vehicle for Heterologous Protein Expression and Intranasal Immunization of Pigs"],"prefix":"10.1128","volume":"70","author":[{"given":"Yoshihiro","family":"Shimoji","sequence":"first","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki 305-0856"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Oishi","sequence":"additional","affiliation":[{"name":"Kyoto Biken Laboratories, Uji, Kyoto 611-0041, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kitajima","sequence":"additional","affiliation":[{"name":"Kyoto Biken Laboratories, Uji, Kyoto 611-0041, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Muneta","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki 305-0856"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinya","family":"Shimizu","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki 305-0856"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Mori","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki 305-0856"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.10.5013"},{"key":"e_1_3_2_3_2","first-page":"11","year":"2000","unstructured":"Fischetti, V. 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