{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:05:57Z","timestamp":1793235957083,"version":"4.5.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            has been shown to require flagella for infection of the stomach. To analyze whether flagella themselves or motility is needed by these pathogens, we constructed flagellated nonmotile mutants. This was accomplished by using both an insertion mutant and an in-frame deletion of the\n            <jats:italic>motB<\/jats:italic>\n            gene. In vitro, these mutants retain flagella (Fla\n            <jats:sup>+<\/jats:sup>\n            ) but are nonmotile (Mot\n            <jats:sup>\u2212<\/jats:sup>\n            ). By using FVB\/N mice, we found that these mutants had reduced ability to infect mice in comparison to that of their isogenic wild-type counterparts. When these mutants were coinfected with wild type, we were unable to detect any\n            <jats:italic>motB<\/jats:italic>\n            mutant. Finally, by analyzing the 50% infectious dose, we found that motility is needed for initial colonization of the stomach mucosa. These results support a model in which motility is used for the initial colonization of the stomach and also to attain full infection levels.\n          <\/jats:p>","DOI":"10.1128\/iai.70.4.1984-1990.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1984-1990","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":284,"title":["<i>Helicobacter pylori<\/i>\n            Uses Motility for Initial Colonization and To Attain Robust Infection"],"prefix":"10.1128","volume":"70","author":[{"given":"Karen M.","family":"Ottemann","sequence":"first","affiliation":[{"name":"Departments of Environmental Toxicology and Molecular, Cell and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew C.","family":"Lowenthal","sequence":"additional","affiliation":[{"name":"Departments of Environmental Toxicology and Molecular, Cell and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90515-4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/16495"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.12.2789-2793.1989"},{"key":"e_1_3_2_5_2","unstructured":"Ausubel F. 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