{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:36:21Z","timestamp":1792665381020,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Using a transposon mutagenesis approach, we have identified a mutant of\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    that is auxotrophic for branched chain amino acids. The transposon was shown to have interrupted the\n                    <jats:italic>ilvI<\/jats:italic>\n                    gene encoding the large subunit of the acetolactate synthase enzyme. Compared to the wild type, this mutant was significantly attenuated in a murine model of disease. Mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were protected against a challenge dose of 6,000 median lethal doses of wild-type\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/iai.70.9.5290-5294.2002","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T15:36:19Z","timestamp":1033486579000},"page":"5290-5294","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":102,"title":["A Mutant of\n                    <i>Burkholderia pseudomallei<\/i>\n                    , Auxotrophic in the Branched Chain Amino Acid Biosynthetic Pathway, Is Attenuated and Protective in a Murine Model of Melioidosis"],"prefix":"10.1128","volume":"70","author":[{"given":"T.","family":"Atkins","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. G.","family":"Prior","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Mack","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Russell","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. C. F.","family":"Oyston","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Dougan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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