{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:23Z","timestamp":1791942383070,"version":"4.3.4"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Expression of the\n            <jats:italic>virB<\/jats:italic>\n            operon, encoding the type IV secretion system required for\n            <jats:italic>Brucella suis<\/jats:italic>\n            virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic pH values. To analyze the production of VirB proteins, polyclonal antisera against\n            <jats:italic>B. suis<\/jats:italic>\n            VirB5 and VirB8 were generated. Western blot analysis revealed that VirB5 and VirB8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. Unlike what occurs in the related organism\n            <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n            , the periplasmic sugar binding protein ChvE did not contribute to VirB protein production, and\n            <jats:italic>B. suis<\/jats:italic>\n            from which\n            <jats:italic>chvE<\/jats:italic>\n            was deleted was fully virulent in a mouse model. Comparative analyses of various\n            <jats:italic>Brucella<\/jats:italic>\n            species revealed that in all of them VirB protein production increased under acidic conditions. However, in rich medium at neutral pH,\n            <jats:italic>Brucella canis<\/jats:italic>\n            and\n            <jats:italic>B. suis<\/jats:italic>\n            , as well as the\n            <jats:italic>Brucella abortus-<\/jats:italic>\n            and\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            -derived vaccine strains S19, RB51, and Rev.1, produced no VirB proteins or only small amounts of VirB proteins, whereas the parental\n            <jats:italic>B. abortus<\/jats:italic>\n            and\n            <jats:italic>B. melitensis<\/jats:italic>\n            strains constitutively produced VirB5 and VirB8. Thus, the vaccine strains were still able to induce\n            <jats:italic>virB<\/jats:italic>\n            expression under acidic conditions, but the VirB protein production was markedly different from that in the wild-type strains at pH 7. Taken together, the data indicate that VirB protein production and probably expression of the\n            <jats:italic>virB<\/jats:italic>\n            operon are not uniformly regulated in different\n            <jats:italic>Brucella<\/jats:italic>\n            species. Since VirB proteins were shown to modulate\n            <jats:italic>Brucella<\/jats:italic>\n            phagocytosis and intracellular trafficking, the differential regulation of the production of these proteins reported here may provide a clue to explain their role(s) during the infection process.\n          <\/jats:p>","DOI":"10.1128\/iai.71.3.1075-1082.2003","type":"journal-article","created":{"date-parts":[[2003,2,20]],"date-time":"2003-02-20T21:48:38Z","timestamp":1045777718000},"page":"1075-1082","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Production of the Type IV Secretion System Differs among\n            <i>Brucella<\/i>\n            Species as Revealed with VirB5- and VirB8-Specific Antisera"],"prefix":"10.1128","volume":"71","author":[{"given":"Bruno","family":"Rouot","sequence":"first","affiliation":[{"name":"INSERM U431, Universite\u0301 de Montpellier 2, 34095 Montpellier Cedex 05"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Teresa","family":"Alvarez-Martinez","sequence":"additional","affiliation":[{"name":"INSERM U431, Universite\u0301 de Montpellier 2, 34095 Montpellier Cedex 05"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Marius","sequence":"additional","affiliation":[{"name":"INSERM U431, Universite\u0301 de Montpellier 2, 34095 Montpellier Cedex 05"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierrette","family":"Menanteau","sequence":"additional","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, INRA, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Guilloteau","sequence":"additional","affiliation":[{"name":"Laboratoire de Pathologie Infectieuse et Immunologie, INRA, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose-Anne","family":"Boigegrain","sequence":"additional","affiliation":[{"name":"INSERM U431, Universite\u0301 de Montpellier 2, 34095 Montpellier Cedex 05"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Zumbihl","sequence":"additional","affiliation":[{"name":"INSERM U431, Universite\u0301 de Montpellier 2, 34095 Montpellier Cedex 05"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"O'Callaghan","sequence":"additional","affiliation":[{"name":"INSERM U431, Universite\u0301 de Montpellier 2, 34095 Montpellier Cedex 05"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Domke","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-Universita\u0308t, Department Biologie I, Bereich Mikrobiologie, D-80638 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baron","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-Universita\u0308t, Department Biologie I, Bereich Mikrobiologie, D-80638 Munich, Germany"},{"name":"Department of Biology, McMaster University, Hamilton, Ontario LS8 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5343-5351.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/24094"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4255-4263.2000"},{"key":"e_1_3_2_5_2","unstructured":"Ausubel S. 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