{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:19:32Z","timestamp":1774563572612,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The use of DNA constructs encoding mycobacterial proteins is a promising new approach to vaccination against tuberculosis. A DNA vaccine encoding the hsp60 molecule of<jats:italic>Mycobacterium leprae<\/jats:italic>has previously been shown to protect against intravenous infection of mice with<jats:italic>Mycobacterium tuberculosis<\/jats:italic>in both the prophylactic and immunotherapeutic modes. It is shown here, however, that this vaccine was not effective in a more realistic aerosol infection model or in a model of latent tuberculosis in the lungs. Moreover, when given in an immunotherapeutic model the immunized mice developed classical Koch reactions characterized by multifocal discrete regions of cellular necrosis throughout the lung granulomas. Similar and equally severe reactions were seen in mice given a vaccine with DNA coding for the Ag85 antigen of<jats:italic>M. tuberculosis<\/jats:italic>. This previously unanticipated safety problem indicates that DNA vaccines should be used with caution in individuals who may have already been exposed to tuberculosis.<\/jats:p>","DOI":"10.1128\/iai.71.4.2192-2198.2003","type":"journal-article","created":{"date-parts":[[2003,3,24]],"date-time":"2003-03-24T23:07:27Z","timestamp":1048547247000},"page":"2192-2198","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Pulmonary Necrosis Resulting from DNA Vaccination against Tuberculosis"],"prefix":"10.1128","volume":"71","author":[{"given":"Jennifer L.","family":"Taylor","sequence":"first","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver C.","family":"Turner","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall J.","family":"Basaraba","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John T.","family":"Belisle","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kris","family":"Huygen","sequence":"additional","affiliation":[{"name":"Pasteur Institute, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian M.","family":"Orme","sequence":"additional","affiliation":[{"name":"Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.6.2951-2959.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1285"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1994.03510330076038"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1231-1234.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.282.7.677"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.93"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.4049\/jimmunol.160.4.1796","volume":"160","year":"1998","unstructured":"Flynn, J. L., C. A. Scanga, K. E. Tanaka, and J. Chan. 1998. Effects of aminoguanidine on latent murine tuberculosis. J. Immunol.160:1796-1803.","journal-title":"J. Immunol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0896-893"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.7.2880-2886.1992"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170308"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1206198"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/22326"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2100-2107.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(99)01594-4"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.6.1481"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01949-1"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3318-3323.2002"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8479(97)90016-2"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4531-4538.1999"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2400-2407.1996"},{"key":"e_1_3_2_22_2","first-page":"200","volume":"2","year":"1998","unstructured":"Sterne, J. A., L. C. Rodrigues, and I. N. Guedes. 1998. Does the efficacy of BCG decline with time since vaccination? Int. J. Tuberc. Lung Dis.2:200-207.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_23_2","first-page":"69","volume":"62","year":"1987","unstructured":"Tripathy, S. P. 1987. Fifteen year follow-up of the Indian BCG Prevention trial. Bull. Int. Union Tubercle Lung Dis.62:69-72.","journal-title":"Bull. Int. Union Tubercle Lung Dis."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1706-1709.2000"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3674-3679.2000"},{"key":"e_1_3_2_26_2","unstructured":"Ulmer J. B. R. R. Deck C. M. DeWitt J. J. Donnelly A. Friedman D. L. Montgomery A. M. Yawman I. M. Orme O. Denis J. Content K. Huygen and M. A. Liu. 1997. Induction of immunity by DNA vaccination: application to influenza and tuberculosis. Behring Inst. Mitt. 98: 79-86."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00271878"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/11.Supplement_2.S484"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.9.3947-3950.1997"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.71.4.2192-2198.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.71.4.2192-2198.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:19:05Z","timestamp":1682468345000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.71.4.2192-2198.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1128\/IAI.71.4.2192-2198.2003"],"URL":"https:\/\/doi.org\/10.1128\/iai.71.4.2192-2198.2003","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]},"assertion":[{"value":"2002-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}