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Consistent with gene-predicted roles, electrophoretic analysis, 2-keto-3-\n            <jats:italic>manno<\/jats:italic>\n            <jats:sc>-d-<\/jats:sc>\n            octulosonate measurements, and immunoblots with monoclonal antibodies to O-polysaccharide, outer and inner core epitopes showed no O-polysaccharide expression and no LPS core defects in the\n            <jats:italic>wbk<\/jats:italic>\n            mutants. The rough LPS of\n            <jats:italic>manB<\/jats:italic>\n            mutant lacked the outer core epitope and the gene was designated\n            <jats:italic>\n              manB\n              <jats:sub>core<\/jats:sub>\n            <\/jats:italic>\n            to distinguish it from the\n            <jats:italic>\n              wbk manB\n              <jats:sub>O-Ag<\/jats:sub>\n            <\/jats:italic>\n            . The fourth gene (provisionally designated\n            <jats:italic>wa**<\/jats:italic>\n            ) coded for a putative glycosyltransferase involved in inner core synthesis, but the mutant kept the outer core epitope. Differences in phage and polymyxin sensitivity, exposure or expression of outer membrane protein, core and lipid A epitopes, and lipid A acylation demonstrated that small changes in LPS core caused significant differences in\n            <jats:italic>B. abortus<\/jats:italic>\n            outer membrane topology. In mice, the mutants showed different degrees of attenuation and induced antibodies to rough LPS and outer membrane proteins. Core-defective mutants and strain RB51 were ineffective vaccines against\n            <jats:italic>B. abortus<\/jats:italic>\n            in mice. The mutants\n            <jats:italic>per<\/jats:italic>\n            and\n            <jats:italic>wbkA<\/jats:italic>\n            induced protection but less than the standard smooth vaccine S19, and controls suggested that anti O-polysaccharide antibodies accounted largely for the difference. Whereas no core-defective mutant was effective against\n            <jats:italic>B. ovis<\/jats:italic>\n            , S19, RB51, and the\n            <jats:italic>wbkA<\/jats:italic>\n            and\n            <jats:italic>per<\/jats:italic>\n            mutants afforded similar levels of protection. These results suggest that rough\n            <jats:italic>Brucella<\/jats:italic>\n            vaccines should carry a complete core for maximal effectiveness.\n          <\/jats:p>","DOI":"10.1128\/iai.71.6.3261-3271.2003","type":"journal-article","created":{"date-parts":[[2003,5,21]],"date-time":"2003-05-21T23:33:12Z","timestamp":1053559992000},"page":"3261-3271","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":98,"title":["Characterization of\n            <i>Brucella abortus<\/i>\n            O-Polysaccharide and Core Lipopolysaccharide Mutants and Demonstration that a Complete Core Is Required for Rough Vaccines To Be Efficient against\n            <i>Brucella abortus<\/i>\n            and\n            <i>Brucella ovis<\/i>\n            in the Mouse Model"],"prefix":"10.1128","volume":"71","author":[{"given":"D.","family":"Monreal","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad de Navarra, Pamplona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Grillo\u0301","sequence":"additional","affiliation":[{"name":"Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad de Navarra, Pamplona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. M.","family":"Mari\u0301n","sequence":"additional","affiliation":[{"name":"Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"De Miguel","sequence":"additional","affiliation":[{"name":"Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Lo\u0301pez-Gon\u0303i","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad de Navarra, Pamplona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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