{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:33:41Z","timestamp":1791272021440,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Apical membrane antigen 1 (AMA-1) of\n            <jats:italic>Plasmodium<\/jats:italic>\n            merozoites is established as a candidate molecule for inclusion in a human malaria vaccine and is strongly conserved in the genus. We have investigated its function in merozoite invasion by incubating\n            <jats:italic>Plasmodium knowlesi<\/jats:italic>\n            merozoites with red cells in the presence of a previously described rat monoclonal antibody (MAb R31C2) raised against an invasion-inhibitory epitope of\n            <jats:italic>P. knowlesi<\/jats:italic>\n            AMA-1 and then fixing the material for ultrastructural analysis. We have found that the random, initial, long-range (12 nm) contact between merozoites and red cells occurs normally in the presence of the antibody, showing that AMA-1 plays no part in this stage of attachment. Instead, inhibited merozoites fail to reorientate, so they do not bring their apices to bear on the red cell surface and do not make close junctional apical contact. We conclude that AMA-1 may be directly responsible for reorientation or that the molecule may initiate the junctional contact, which is then presumably dependent on Duffy binding proteins for its completion.\n          <\/jats:p>","DOI":"10.1128\/iai.72.1.154-158.2004","type":"journal-article","created":{"date-parts":[[2003,12,20]],"date-time":"2003-12-20T02:48:22Z","timestamp":1071888502000},"page":"154-158","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":188,"title":["Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells"],"prefix":"10.1128","volume":"72","author":[{"given":"G. H.","family":"Mitchell","sequence":"first","affiliation":[{"name":"Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London SE1 9RT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. W.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Biomedical Primate Research Centre, 2280 GH, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Margos","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London SE1 9RT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. R.","family":"Dluzewski","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London SE1 9RT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. H.","family":"Bannister","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Cell and Human Biology, Guy's, King's and St. Thomas' School of Biomedical Science, Guy's Hospital, London SE1 1UL, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90295-P"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.15.7085"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.77.1.72"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00086-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000047247"},{"key":"e_1_3_2_7_2","first-page":"5","volume":"69","year":"1975","unstructured":"Bannister, L. H., G. A. Butcher, E. D. Dennis, and G. H. Mitchell. 1975. Studies on the structure and invasive behaviour of merozoites of Plasmodium knowlesi. Trans. R. 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