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Because of the potential of DNA vaccines for immunotherapy of chronic and infectious diseases, we tested if DNA vaccines could control an ongoing<jats:italic>Trypanosoma cruzi<\/jats:italic>infection. BALB\/c mice were infected with a lethal dose (5 \u00d7 10<jats:sup>4<\/jats:sup>parasites) as a model of acute infection, and then they were treated with two injections of 100 \u03bcg of plasmid DNA 1 week apart, beginning on day 5 postinfection. Control mice had high levels of parasitemia and mortality and severe cardiac inflammation, while mice treated with plasmid DNA encoding trypomastigote surface antigen 1 or Tc24 had reduced parasitemia and mild cardiac inflammation and &gt;70% survived the infection. The efficacy of the immunotherapy also was significant when it was delayed until days 10 and 15 after infection. Parasitological analysis of cardiac tissue of surviving mice indicated that most mice still contained detectable parasite kinetoplast DNA but fewer mice contained live parasites, suggesting that there was efficient but not complete parasite elimination. DNA vaccine immunotherapy was also evaluated in CD1 mice infected with a low dose (5 \u00d7 10<jats:sup>2<\/jats:sup>parasites) as a model of chronic infection. Immunotherapy was initiated on day 70 postinfection and resulted in improved survival and reduced cardiac tissue inflammation. These results suggest that DNA vaccines have strong potential for the immunotherapy of<jats:italic>T. cruzi<\/jats:italic>infection and may provide new alternatives for the control of Chagas' disease.<\/jats:p>","DOI":"10.1128\/iai.72.1.46-53.2004","type":"journal-article","created":{"date-parts":[[2003,12,20]],"date-time":"2003-12-20T02:48:22Z","timestamp":1071888502000},"page":"46-53","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Immunotherapy of<i>Trypanosoma cruzi<\/i>Infection with DNA Vaccines in Mice"],"prefix":"10.1128","volume":"72","author":[{"given":"Eric","family":"Dumonteil","sequence":"first","affiliation":[{"name":"Laboratorio de Parasitologi\u0301a, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Auto\u0301noma de Yucata\u0301n, Me\u0301rida, Yucata\u0301n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Escobedo-Ortegon","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitologi\u0301a, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Auto\u0301noma de Yucata\u0301n, Me\u0301rida, Yucata\u0301n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"Reyes-Rodriguez","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitologi\u0301a, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Auto\u0301noma de Yucata\u0301n, Me\u0301rida, Yucata\u0301n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arletty","family":"Arjona-Torres","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitologi\u0301a, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Auto\u0301noma de Yucata\u0301n, Me\u0301rida, Yucata\u0301n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jesus","family":"Ramirez-Sierra","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitologi\u0301a, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Auto\u0301noma de Yucata\u0301n, Me\u0301rida, Yucata\u0301n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2001.0523"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2494(91)90021-A"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.726"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.32776\/revbiomed.v12i4.279"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5273(97)00083-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/6995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/514148"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00277-6"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.740"},{"key":"e_1_3_2_11_2","first-page":"S7","volume":"11","year":"2001","unstructured":"Dumonteil, E. 2001. 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