{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T12:40:13Z","timestamp":1793968813519,"version":"4.8.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Feto-placental infections represent a major cause of pregnancy complications, and yet the underlying molecular and cellular mechanisms of vertical transmission are poorly understood.\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    , a facultative intracellular pathogen, is one of a group of pathogens that are known to cause feto-placental infections in humans and other mammals. The purpose of this study was to evaluate possible mechanisms of vertical transmission of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    . Humans and guinea pigs have a hemochorial placenta, where a single layer of fetally derived trophoblasts separates maternal from fetal circulation. We characterized\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    infection of the feto-placental unit in a pregnant guinea pig model and in primary human trophoblasts and trophoblast-derived cell lines. The clinical manifestations of listeriosis in the pregnant guinea pigs and the tropism of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    to the guinea pig placenta resembled those in humans. Trophoblast cell culture systems were permissive for listerial growth and cell-to-cell spread and revealed that\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    deficient in internalin A, a virulence factor that mediates invasion of nonphagocytic cells, was 100-fold defective in invasion. However, crossing of the feto-placental barrier in the guinea pig model was independent of internalin A, suggesting a negligible role for internalin-mediated direct invasion of trophoblasts in vivo. Further understanding of vertical transmission of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    will help in designing more effective means of treatment and disease prevention.\n                  <\/jats:p>","DOI":"10.1128\/iai.72.1.489-497.2004","type":"journal-article","created":{"date-parts":[[2003,12,19]],"date-time":"2003-12-19T21:48:22Z","timestamp":1071870502000},"page":"489-497","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":125,"title":["Listeriosis in the Pregnant Guinea Pig: a Model of Vertical Transmission"],"prefix":"10.1128","volume":"72","author":[{"given":"Anna I.","family":"Bakardjiev","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian A.","family":"Stacy","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Service, Veterinary Medical Teaching Hospital, University of California, Davis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Departments of Stomatology"},{"name":"Anatomy"},{"name":"Pharmaceutical Chemistry, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology"},{"name":"School of Public Health, University of California, Berkeley"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5953-5957.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","volume":"139","year":"1987","unstructured":"Bishop, D. 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