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Human T-cell clones (TCC) were isolated from peripheral blood mononuclear cells of five healthy donors with prior cryptosporidiosis by use of a<jats:italic>C. parvum<\/jats:italic>crude extract, two antigen fractions obtained by ion-exchange chromatography (IEC1 and IEC2), and two recombinant peptides (SA35 and SA40) from<jats:italic>C. parvum<\/jats:italic>sporozoites. The T-cell lines derived from the one recently infected donor had a higher proportion (26 to 38%) of T cells exhibiting the \u03b3\/\u03b4 T-cell receptor (\u03b3\/\u03b4-TCR) than those from donors who had recovered from cryptosporidiosis several years earlier, suggesting that the \u03b3\/\u03b4 T-cell population is involved in the early stage of the infection. The specific TCC had the \u03b1\/\u03b2-TCR, had the phenotype CD45RO<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>, and were characterized by either hyperproduction of gamma interferon (IFN-\u03b3) alone, with a Th1 profile, or IFN-\u03b3 hyperproduction together with interleukin-4 (IL-4) or IL-5 production, with a Th0 profile. SA35, SA40, IEC1, and IEC2 may be considered good targets of the cellular response against<jats:italic>C. parvum<\/jats:italic>and may play a role in maintaining the T-cell-mediated memory response to this parasite. Furthermore, the SA35 and SA40 peptides may be regarded as immunodominant antigens involved in the maintenance of the T-cell response in healthy<jats:italic>C. parvum<\/jats:italic>-sensitized persons.<\/jats:p>","DOI":"10.1128\/iai.72.3.1306-1310.2004","type":"journal-article","created":{"date-parts":[[2004,2,20]],"date-time":"2004-02-20T23:42:58Z","timestamp":1077320578000},"page":"1306-1310","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["<i>Cryptosporidium parvum<\/i>-Specific CD4 Th1 Cells from Sensitized Donors Responding to Both Fractionated and Recombinant Antigenic Proteins"],"prefix":"10.1128","volume":"72","author":[{"given":"Maria Angeles Gomez","family":"Morales","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, 00161 Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaella","family":"Mele","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, 00161 Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Ludovisi","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, 00161 Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Bruschi","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Tosini","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, 00161 Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo","family":"Pozio","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300, 00161 Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.2.697-699.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11401-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(98)00119-4"},{"key":"e_1_3_2_5_2","first-page":"813","volume":"85","year":"1992","unstructured":"Blanshard, C., A. 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