{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:29:09Z","timestamp":1787016549421,"version":"build-2736575974"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The protective efficacy of\n                    <jats:italic>Mycobacterium bovis<\/jats:italic>\n                    BCG can be markedly augmented by stable integration of\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    genomic region RD1. BCG complemented with RD1 (BCG::RD1) encodes nine additional proteins. Among them, 10-kDa culture filtrate protein (CFP-10) and ESAT-6 (6-kDa early secreted antigenic target) are low-molecular-weight proteins that induce potent Th1 responses. Using pools of synthetic peptides, we have examined the potential immunogenicity of four other RD1 products (PE35, PPE68, Rv3878, and Rv3879c). PPE68, the protein encoded by\n                    <jats:italic>rv3873<\/jats:italic>\n                    , was the only one to elicit gamma interferon (IFN-\u03b3)-producing cells in C57BL\/6 mice infected with\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    . Anti-PPE68 T cells were predominantly raised against an epitope mapped in the N-terminal end of the protein. Importantly, inactivation of\n                    <jats:italic>rv3873<\/jats:italic>\n                    in BCG::RD1 did not modify CFP-10 and ESAT-6 secretion. Moreover, the generation of IFN-\u03b3 responses to these antigens following immunization with BCG::RD1 was independent of PPE68 expression. Taken together, these results show that PPE68 is an immunogenic product of the RD1 region, which does not interfere with the secretion and immunogenicity of CFP-10 and ESAT-6.\n                  <\/jats:p>","DOI":"10.1128\/iai.72.4.2170-2176.2004","type":"journal-article","created":{"date-parts":[[2004,3,23]],"date-time":"2004-03-23T17:43:10Z","timestamp":1080063790000},"page":"2170-2176","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Cell Envelope Protein PPE68 Contributes to\n                    <i>Mycobacterium tuberculosis<\/i>\n                    RD1 Immunogenicity Independently of a 10-Kilodalton Culture Filtrate Protein and ESAT-6"],"prefix":"10.1128","volume":"72","author":[{"given":"Caroline","family":"Demangel","sequence":"first","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire Bacte\u0301rienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscille","family":"Brodin","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire Bacte\u0301rienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Cockle","sequence":"additional","affiliation":[{"name":"VLA Weybridge, Department of Bacterial Diseases, TB Research Group, Addlestone, Surrey, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Brosch","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire Bacte\u0301rienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laleh","family":"Majlessi","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Biologie des Re\u0301gulations Immunitaires, INSERM E352, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Leclerc","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Biologie des Re\u0301gulations Immunitaires, INSERM E352, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart T.","family":"Cole","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire Bacte\u0301rienne"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3719-3728.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.3.551"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/315448"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.791-795.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.4049\/jimmunol.157.8.3527","volume":"157","year":"1996","unstructured":"Brandt, L., T. 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Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J. E. Sulston, K. Taylor, S. Whitehead, and B. G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. 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