{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:51:11Z","timestamp":1789930271801,"version":"4.0.1"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Pathogenic\n            <jats:italic>Brucella<\/jats:italic>\n            species replicate within mammalian cells, and their type IV secretion system is essential for intracellular survival and replication. The options for biochemical studies on the\n            <jats:italic>Brucella<\/jats:italic>\n            secretion system are limited due to the rigidity of the cells and biosafety concerns, which preclude large-scale cell culture and fractionation. To overcome these problems, we heterologously expressed the\n            <jats:italic>Brucella suis virB<\/jats:italic>\n            operon in the closely related \u03b1\n            <jats:sub>2<\/jats:sub>\n            -proteobacterium\n            <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n            and showed that the VirB proteins assembled into a complex. Eight of the twelve VirB proteins were detected in the membranes of the heterologous host with specific antisera. Cross-linking indicated protein-protein interactions similar to those in other type IV secretion systems, and the results of immunofluorescence analysis supported the formation of VirB protein complexes in the cell envelope. Production of a subset of the\n            <jats:italic>B. suis<\/jats:italic>\n            VirB proteins (VirB3-VirB12) in\n            <jats:italic>A. tumefaciens<\/jats:italic>\n            strongly increased its ability to receive IncQ plasmid pLS1 in conjugation experiments, and production of VirB1 further enhanced the conjugation efficiency. Plasmid recipient competence correlated with periplasmic leakage and the detergent sensitivity of\n            <jats:italic>A. tumefaciens<\/jats:italic>\n            , suggesting a weakening of the cell envelope. Heterologous expression thus permits biochemical characterization of\n            <jats:italic>B. suis<\/jats:italic>\n            type IV secretion system assembly.\n          <\/jats:p>","DOI":"10.1128\/iai.74.1.108-117.2006","type":"journal-article","created":{"date-parts":[[2005,12,20]],"date-time":"2005-12-20T22:02:58Z","timestamp":1135116178000},"page":"108-117","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["The\n            <i>Brucella suis<\/i>\n            Type IV Secretion System Assembles in the Cell Envelope of the Heterologous Host\n            <i>Agrobacterium tumefaciens<\/i>\n            and Increases IncQ Plasmid pLS1 Recipient Competence"],"prefix":"10.1128","volume":"74","author":[{"given":"Anna","family":"Carle","sequence":"first","affiliation":[{"name":"Department Biologie I, Bereich Mikrobiologie, Ludwig-Maximilians-Universita\u0308t, Maria-Ward-Str. 1a, D-80638 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Ho\u0308ppner","sequence":"additional","affiliation":[{"name":"Department Biologie I, Bereich Mikrobiologie, Ludwig-Maximilians-Universita\u0308t, Maria-Ward-Str. 1a, D-80638 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled","family":"Ahmed Aly","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main St. West, Hamilton, Ontario LS8 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main St. West, Hamilton, Ontario LS8 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amke","family":"den Dulk-Ras","sequence":"additional","affiliation":[{"name":"Clusius Laboratory, Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Vergunst","sequence":"additional","affiliation":[{"name":"Clusius Laboratory, Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands"},{"name":"Unite\u0301 431, UFR de Me\u0301decine, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, CS 83021, Avenue J. F. Kennedy, 30908 Ni\u0302mes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"O'Callaghan","sequence":"additional","affiliation":[{"name":"Unite\u0301 431, UFR de Me\u0301decine, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, CS 83021, Avenue J. F. Kennedy, 30908 Ni\u0302mes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baron","sequence":"additional","affiliation":[{"name":"Department Biologie I, Bereich Mikrobiologie, Ludwig-Maximilians-Universita\u0308t, Maria-Ward-Str. 1a, D-80638 Munich, Germany"},{"name":"Department of Biology, McMaster University, 1280 Main St. West, Hamilton, Ontario LS8 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2002.0514"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.4.1203-1210.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.4.1211-1218.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.10.3176-3183.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.1.78-89.1997"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.12.3646-3660.1994"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.7057"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00165-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032514299"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095211"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro753"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2003.11.001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406873102"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.02.013"},{"key":"e_1_3_2_16_2","first-page":"415","volume":"59","year":"2005","unstructured":"Christie, P. 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