{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:19:37Z","timestamp":1778775577206,"version":"3.51.4"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Brucella abortus<\/jats:italic>is a facultative, intracellular zoonotic pathogen which can cause undulant fever in humans and abortions in cattle. A 14-kDa protein of<jats:italic>B. abortus<\/jats:italic>was previously identified to be immunogenic in animals infected with<jats:italic>Brucella<\/jats:italic>spp. In this study, we discovered that the 14-kDa protein possessed immunoglobulin binding and hemagglutination properties that appeared to be based on the protein's lectin-like properties. Hemagglutination inhibition experiments suggested that the 14-kDa protein has affinity towards mannose. Disruption of the gene encoding the 14-kDa protein in virulent<jats:italic>B. abortus<\/jats:italic>strain 2308 induced a rough-like phenotype with an altered smooth lipopolysaccharide (LPS) immunoblot profile and a significant reduction in the bacterium's ability to replicate in mouse spleens. However, the mutant strain was stably maintained in mouse spleens at 2.0 to 2.6 log<jats:sub>10<\/jats:sub>CFU\/spleen from day 1 to week 6 after intraperitoneal inoculation with 4.65 log<jats:sub>10<\/jats:sub>CFU. In contrast to the case for the smooth virulent strain 2308, in the rough attenuated strain RB51 disruption of the 14-kDa protein's gene had no effect on the mouse clearance pattern. These findings indicate that the 14-kDa protein of<jats:italic>B. abortus<\/jats:italic>possesses lectin-like properties and is essential for the virulence of the species, probably because of its direct or indirect role in the synthesis of smooth LPS.<\/jats:p>","DOI":"10.1128\/iai.74.1.183-191.2006","type":"journal-article","created":{"date-parts":[[2005,12,20]],"date-time":"2005-12-20T22:02:58Z","timestamp":1135116178000},"page":"183-191","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Role in Virulence of a<i>Brucella abortus<\/i>Protein Exhibiting Lectin-Like Activity"],"prefix":"10.1128","volume":"74","author":[{"given":"Tracy H.","family":"Vemulapalli","sequence":"first","affiliation":[{"name":"Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Vemulapalli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhardt G.","family":"Schurig","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nammalwar","family":"Sriranganathan","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.1008-1016.1998"},{"key":"e_1_3_1_3_2","unstructured":"Alton G. G. L. M. Jones and D. E. Pietz. 1975. Laboratory techniques in brucellosis. World Health Organization monograph series no. 55. World Health Organization Geneva Switzerland."},{"key":"e_1_3_1_4_2","unstructured":"Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1993. Current protocols in molecular biology vol. 2. Greene Publishing Associates and Wiley-Interscience New York N.Y."},{"key":"e_1_3_1_5_2","first-page":"363","volume":"60","year":"1994","unstructured":"Baldwin, C. L., and A. J. Winter. 1994. Macrophages and Brucella. Immunol. Semin.60:363-380.","journal-title":"Immunol. Semin."},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/0307945021000024526"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.8.4000-4003.1998"},{"key":"e_1_3_1_8_2","first-page":"377","volume":"1","year":"1984","unstructured":"Corbel, M. J., and W. J. Brinley-Morgan. 1984. Genus Brucella, p. 377-388. In N. R. Krieg (ed.), Bergey's manual of systematic bacteriology, vol. 1. Williams & Wilkins, Baltimore, Md.","journal-title":"Bergey's manual of systematic bacteriology"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11309.x"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098589002700503"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.7.2320-2328.1990"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00236-5"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb06828.x"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00214-6"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.1.65-78.2003"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00584-1"},{"key":"e_1_3_1_17_2","first-page":"800","volume":"16","year":"1994","unstructured":"Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Roop II, and K. M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques16:800-802.","journal-title":"BioTechniques"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.3830-3835.1999"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2005.03.002"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3261-3271.2003"},{"key":"e_1_3_1_21_2","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.4049\/jimmunol.146.4.1247","volume":"146","year":"1991","unstructured":"Raeder, R., E. L. Faulmann, and M. D. Boyle. 1991. Evidence for functional heterogeneity in IgG Fc-binding proteins associated with group A streptococci. J. Immunol.146:1247-1253.","journal-title":"J. Immunol."},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501130"},{"key":"e_1_3_1_23_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90091-S"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00255-9"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2001.tb00580.x"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6264-6269.2003"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3286-3289.2000"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3290-3296.2000"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.3927-3932.2000"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.1.183-191.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.1.183-191.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T23:27:07Z","timestamp":1683329227000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.74.1.183-191.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,1]]}},"alternative-id":["10.1128\/IAI.74.1.183-191.2006"],"URL":"https:\/\/doi.org\/10.1128\/iai.74.1.183-191.2006","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1]]},"assertion":[{"value":"2005-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-09-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}