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Clinical and experimental studies indicate that prior symptomatic coccidioidomycosis induces immunity against subsequent infection, and activation of T cells is essential for containment of the pathogen and its clearance from host tissue. Development of a human vaccine against coccidioidomycosis has focused on recombinant T-cell-reactive antigens which elicit a durable protective immune response against pulmonary infection in mice. In this study we fractionated a protective multicomponent parasitic cell wall extract in an attempt to identify T-cell antigens. Immunoblots of electrophoretic separations of this extract identified patient seroreactive proteins which were subsequently excised from two-dimensional polyacrylamide gel electrophoresis gels, trypsin digested, and sequenced by tandem mass spectrometry. The full-length gene which encodes a dominant protein in the immunoblot was identified using established methods of bioinformatics. The gene was cloned and expressed, and the recombinant protein was shown to stimulate immune T cells in vitro. The deduced protein was predicted to contain epitopes that bind to human major histocompatibility complex class II molecules using a TEPITOPE-based algorithm. Synthetic peptides corresponding to the predicted T-cell epitopes induced gamma interferon production by immune T lymphocytes. The T-cell-reactive antigen, which is homologous to secreted fungal aspartyl proteases, protected mice against pulmonary infection with<jats:italic>Coccidioides posadasii<\/jats:italic>. We argue that this immunoproteomic\/bioinformatic approach to the identification of candidate vaccines against coccidioidomycosis is both efficient and productive.<\/jats:p>","DOI":"10.1128\/iai.74.1.516-527.2006","type":"journal-article","created":{"date-parts":[[2005,12,20]],"date-time":"2005-12-20T22:02:58Z","timestamp":1135116178000},"page":"516-527","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["A Recombinant Aspartyl Protease of<i>Coccidioides posadasii<\/i>Induces Protection against Pulmonary Coccidioidomycosis in Mice"],"prefix":"10.1128","volume":"74","author":[{"given":"Eric J.","family":"Tarcha","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio 43614"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatesha","family":"Basrur","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio 43614"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiung-Yu","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio 43614"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm J.","family":"Gardner","sequence":"additional","affiliation":[{"name":"The Institute for Genomic Research, Rockville, Maryland 20850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garry T.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio 43614"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/430303"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2004.11.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0705.010505"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.8.4731"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.10.2695-2701.1988"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.10.2686-2694.1988"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780410001687349"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2494(98)80765-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01409-5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.01.059"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/1369378031000156890"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3010-3019.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti299"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2003.11833250"},{"key":"e_1_3_2_17_2","first-page":"134","volume":"32","year":"2004","unstructured":"Hulo, N., C. 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