{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:25:22Z","timestamp":1791275122512,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cryptosporidiosis in humans is caused by the zoonotic pathogen\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            and the anthroponotic pathogen\n            <jats:italic>Cryptosporidium hominis<\/jats:italic>\n            . To what extent the recently recognized\n            <jats:italic>C. hominis<\/jats:italic>\n            species differs from\n            <jats:italic>C. parvum<\/jats:italic>\n            is unknown. In this study we compared the mechanisms of\n            <jats:italic>C. parvum<\/jats:italic>\n            and\n            <jats:italic>C. hominis<\/jats:italic>\n            invasion using a primary cell model of infection. Cultured primary bovine and human epithelial intestinal cells were infected with\n            <jats:italic>C. parvum<\/jats:italic>\n            or\n            <jats:italic>C. hominis<\/jats:italic>\n            . The effects of the carbohydrate lectin galactose-\n            <jats:italic>N<\/jats:italic>\n            -acetylgalactosamine (Gal\/GalNAc) and inhibitors of cytoskeletal function and signal transduction mechanisms on entry of the parasites into host cells were tested. HCT-8 cells (human ileocecal adenocarcinoma cells) were used for the purpose of comparison. Pretreatment of parasites with Gal\/GalNAc inhibited entry of\n            <jats:italic>C. parvum<\/jats:italic>\n            into HCT-8 cells and primary bovine cells but had no effect on entry of either\n            <jats:italic>C. parvum<\/jats:italic>\n            or\n            <jats:italic>C. hominis<\/jats:italic>\n            into primary human cells or on entry of\n            <jats:italic>C. hominis<\/jats:italic>\n            into HCT-8 cells. Both\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species entered primary cells by a protein kinase C (PKC)- and actin-dependent mechanism. Staurosporine, in particular, attenuated infection, likely through a combination of PKC inhibition and induction of apoptosis. Diversity in the mechanisms used by\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species to infect cells of different origins has important implications for understanding the relevance of in vitro studies of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/iai.74.1.99-107.2006","type":"journal-article","created":{"date-parts":[[2005,12,20]],"date-time":"2005-12-20T22:02:58Z","timestamp":1135116178000},"page":"99-107","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Interaction of\n            <i>Cryptosporidium hominis<\/i>\n            and\n            <i>Cryptosporidium parvum<\/i>\n            with Primary Human and Bovine Intestinal Cells"],"prefix":"10.1128","volume":"74","author":[{"given":"Amna","family":"Hashim","sequence":"first","affiliation":[{"name":"The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland"},{"name":"UCD School of Medicine and Medical Science"},{"name":"UCD School of Agriculture, Food Science and Veterinary Medicine"},{"name":"UCD Conway Institute of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Mulcahy","sequence":"additional","affiliation":[{"name":"UCD School of Agriculture, Food Science and Veterinary Medicine"},{"name":"UCD Conway Institute of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billy","family":"Bourke","sequence":"additional","affiliation":[{"name":"The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland"},{"name":"UCD School of Medicine and Medical Science"},{"name":"UCD Conway Institute of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marguerite","family":"Clyne","sequence":"additional","affiliation":[{"name":"The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland"},{"name":"UCD School of Medicine and Medical Science"},{"name":"UCD Conway Institute of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094786"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5670-5675.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00138-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109254200"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)00662-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.3011-3021.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)70219-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.24850"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.6806-6816.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00415-3"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"L429","DOI":"10.1152\/ajplung.2000.279.3.L429","volume":"279","year":"2000","unstructured":"Dempsey, E. 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