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Representative cell surface molecules were expressed at substantial levels on Jaws II cells, indicating that appropriate maturation of the cells was achieved with the three<jats:italic>H. pylori<\/jats:italic>antigens without any significant differences.<jats:italic>H. pylori<\/jats:italic>WCS-pulsed Jaws II cells secreted a significant amount of tumor necrosis factor alpha into the culture supernatant. The nai\u0308ve T cells exposed to the WCS-pulsed Jaws II cells showed significant proliferation and gamma interferon (IFN-\u03b3) and interleukin-10 (IL-10) production in vitro. A 2-log reduction in the number of colonizing bacteria was observed in the mice treated with the WCS-pulsed Jaws II cells; however, no significant reductions were achieved in mice treated with Jaws II cells pulsed with other<jats:italic>H. pylori<\/jats:italic>antigens. Up-regulated production of IFN-\u03b3 and IL-10 was observed in the stomachs of the mice treated with the WCS-pulsed Jaws II cells, which is consistent with the result obtained in vitro. There were no differences in gastritis scores or<jats:italic>H. pylori<\/jats:italic>-specific antibody titers among the mice treated with Jaws II cells pulsed with the three different<jats:italic>H. pylori<\/jats:italic>antigens. The results suggest that Th1 cell-mediated immunity in combination with Th2 cell-mediated immunity plays a role in reducing colonizing bacterial numbers in mice with chronic<jats:italic>H. pylori<\/jats:italic>infections.<\/jats:p>","DOI":"10.1128\/iai.74.2.984-993.2006","type":"journal-article","created":{"date-parts":[[2006,1,21]],"date-time":"2006-01-21T01:42:38Z","timestamp":1137807758000},"page":"984-993","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-Term<i>Helicobacter pylori<\/i>Infection in Mice"],"prefix":"10.1128","volume":"74","author":[{"given":"Satoshi","family":"Otsu","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases (Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyo","family":"Gotoh","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases (Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsu","family":"Yamashiro","sequence":"additional","affiliation":[{"name":"Institute of Scientific Research, Faculty of Medicine, Oita University, Oita"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junpei","family":"Yamagata","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases (Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kouichirou","family":"Shin","sequence":"additional","affiliation":[{"name":"Nutritional Science Laboratory, Morinaga Milk Industry Co. 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