{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:31:30Z","timestamp":1759332690613},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Chlamydia<\/jats:italic>-specific cytotoxic T lymphocytes are able to control model infections but may be implicated in disease pathogenesis. HLA-A2 peptide tetramers to<jats:italic>Chlamydia trachomatis<\/jats:italic>major outer membrane protein 258-266 (MOMP258-266) and MOMP260-268 were used to characterize HLA class I-restricted CD8<jats:sup>+<\/jats:sup>T cells in Gambian children aged 4 to 15 years with clinical signs of active trachoma and\/or infection with<jats:italic>C. trachomatis<\/jats:italic>. The frequencies of circulating HLA-A2 tetramer binding cells (TBC) were determined in whole blood samples by flow cytometric analysis. Initial screening of subjects with an anti-HLA-A2 antibody confirmed the presence of either HLA-A2 or HLA-A28. These were subsequently further divided by molecular subtyping. The<jats:italic>C. trachomatis<\/jats:italic>-specific HLA-A2 peptide tetramers were able to bind T cells with receptors from subjects which were restricted by either the HLA-A2 or the HLA-A28 restriction element. In this population, the median value of<jats:italic>C. trachomatis<\/jats:italic>-specific CD8<jats:sup>+<\/jats:sup>T cells was 0.02%, with frequencies of up to 3.71% of CD8<jats:sup>+<\/jats:sup>T cells reactive with a single tetramer in a minority of subjects. TBC were detected more often in subjects who were infected at the ocular surface, and their presence was associated with infection episodes of longer duration. Detection of<jats:italic>C. trachomatis<\/jats:italic>-specific TBC was not associated with the presence of disease or with the estimated load of ocular<jats:italic>C. trachomatis<\/jats:italic>infection at the time of sample collection. High frequencies of<jats:italic>C. trachomatis<\/jats:italic>-specific cells did not predict subsequent appearance or resolution of the clinical disease signs of active trachoma.<\/jats:p>","DOI":"10.1128\/iai.74.3.1565-1572.2006","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T00:18:14Z","timestamp":1140653894000},"page":"1565-1572","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["The Frequency of<i>Chlamydia trachomatis<\/i>Major Outer Membrane Protein-Specific CD8<sup>+<\/sup>T Lymphocytes in Active Trachoma Is Associated with Current Ocular Infection"],"prefix":"10.1128","volume":"74","author":[{"given":"Martin J.","family":"Holland","sequence":"first","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"},{"name":"London School of Hygiene &amp; Tropical Medicine, London WC1E 7HT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nkoyo","family":"Faal","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isatou","family":"Sarr","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Joof","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mass","family":"Laye","sequence":"additional","affiliation":[{"name":"National Eye Care Programme, Kerewan District Health Centre, Kerewan, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewen","family":"Cameron","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick","family":"Pemberton-Pigott","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel M.","family":"Dockrell","sequence":"additional","affiliation":[{"name":"London School of Hygiene &amp; Tropical Medicine, London WC1E 7HT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin L.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratories, Banjul, The Gambia"},{"name":"London School of Hygiene &amp; Tropical Medicine, London WC1E 7HT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C. 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