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In this study we used reverse transcriptase-PCR-differential display (to identify additional GdhR-regulated genes. The results indicated that GdhR, in addition to NADP-GDH, controls the expression of a number of genes involved in glucose catabolism by the Entner-Doudoroff pathway and in<jats:sc>l<\/jats:sc>-glutamate import by an unknown ABC transport system. The genes encoding the putative periplasmic substrate-binding protein (NMB1963) and the permease (NMB1965) of the ABC transporter were genetically inactivated. Uptake experiments demonstrated an impairment of<jats:sc>l<\/jats:sc>-glutamate import in the NMB1965-defective mutant in the absence or in the presence of a low sodium ion concentration. In contrast, at a sodium ion concentration above 60 mM, the uptake defect disappeared, possibly because the activity of a sodium-driven secondary transporter became predominant. Indeed, the NMB1965-defective mutant was unable to grow at a low sodium ion concentration (&lt;20 mM) in a chemically defined medium containing<jats:sc>l<\/jats:sc>-glutamate and four other amino acids that supported meningococcal growth, but it grew when the sodium ion concentration was raised to higher values (&gt;60 mM). The same growth phenotype was observed in the NMB1963-defective mutant. Cell invasion and intracellular persistence assays and expression data during cell invasion provided evidence that the<jats:sc>l<\/jats:sc>-glutamate ABC transporter, tentatively named GltT, was critical for meningococcal adaptation in the low-sodium intracellular environment.<\/jats:p>","DOI":"10.1128\/iai.74.3.1725-1740.2006","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T00:18:14Z","timestamp":1140653894000},"page":"1725-1740","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Identification of a Meningococcal<scp>l<\/scp>-Glutamate ABC Transporter Operon Essential for Growth in Low-Sodium Environments"],"prefix":"10.1128","volume":"74","author":[{"given":"Caterina","family":"Monaco","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita\u0300 di Lecce, Via Monteroni, 73100 Lecce"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adelfia","family":"Tala\u0300","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita\u0300 di Lecce, Via Monteroni, 73100 Lecce"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Rita","family":"Spinosa","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita\u0300 di Lecce, Via Monteroni, 73100 Lecce"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinzia","family":"Progida","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita\u0300 di Lecce, Via Monteroni, 73100 Lecce"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleanna","family":"De Nitto","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita\u0300 di Lecce, Via Monteroni, 73100 Lecce"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Gaballo","sequence":"additional","affiliation":[{"name":"Institute of Biomembranes and Bioenergetics of the C.N.R., Piazza G. 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