{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:34:52Z","timestamp":1784252092406,"version":"3.55.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins LigA and LigB of\n            <jats:italic>Leptospira interrogans<\/jats:italic>\n            . LigA and LigB are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. Here, we evaluate the potential of recombinant LigA (rLigA) as a vaccine candidate against infection by\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovar Pomona in a hamster model. rLigA was truncated into conserved (rLigAcon) and variable (rLigAvar) regions and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            as a fusion protein with glutathione-\n            <jats:italic>S<\/jats:italic>\n            -transferase (rLigA). Golden Syrian hamsters were immunized at 3 and 6 weeks of age with rLigA (rLigAcon and rLigAvar) with aluminum hydroxide as an adjuvant. Hamsters given recombinant glutathione-\n            <jats:italic>S<\/jats:italic>\n            -transferase (rGST)-adjuvant and phosphate-buffered saline-adjuvant served as nonvaccinated controls. Three weeks after the last vaccination, all animals were challenged intraperitoneally with 10\n            <jats:sup>8<\/jats:sup>\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovar Pomona bacteria (NVSL 1427-35-093002). All hamsters immunized with recombinant LigA survived after challenge and had no significant histopathological changes. In contrast, nonimmunized and rGST-immunized hamsters were subjected to lethal doses, and the hamsters that survived showed severe tubulointerstitial nephritis. All vaccinated animals showed a rise in antibody titers against rLigA. Results from this study indicate that rLigA is a potential vaccine candidate against\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovar Pomona infection.\n          <\/jats:p>","DOI":"10.1128\/iai.74.3.1745-1750.2006","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T00:18:14Z","timestamp":1140653894000},"page":"1745-1750","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["Immunoprotection of Recombinant Leptospiral Immunoglobulin-Like Protein A against\n            <i>Leptospira interrogans<\/i>\n            Serovar Pomona Infection"],"prefix":"10.1128","volume":"74","author":[{"given":"Raghavan U. M.","family":"Palaniappan","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P.","family":"McDonough","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Divers","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Sui","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Jeng","family":"Pan","sequence":"additional","affiliation":[{"name":"Institute of Medical Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuharu","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung-Fu","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.14.3.703-708.1976"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.153.6.165"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(99)90029-4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4062-4069.2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6831-6838.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00439-0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00187-5"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3543-3549.1995"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.137.8.192"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01720308"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2414-2421.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4853-4863.2005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1987.tb02615.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879500700114"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879100300208"},{"key":"e_1_3_2_19_2","unstructured":"Faine S. B. Adher C. Bloin and P. Perolat. 1999. Leptospira and leptospirosis 2nd ed. MedSci Melbourne Australia."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-7-1491"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2276-2285.2000"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.4.1579-1587.1998"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6572-6582.1999"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1076\/ocii.6.4.239.4026"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.10.007"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03619.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.70-78.2005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000178824.05715.2c"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7550-7558.2001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.11.6147-6157.2002"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.7014-7017.2005"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.400-404.2001"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45568-0"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.11.5924-5930.2002"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-0233(97)80011-1"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.3174-3181.1995"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.145.19.539"},{"key":"e_1_3_2_39_2","first-page":"722","volume":"184","year":"1984","unstructured":"Thiermann, A. B. 1984. Leptospirosis: current developments and trends. J. Am. Vet. Med. Assoc.184:722-725.","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(01)00281-4"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.3.1745-1750.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.3.1745-1750.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:24:58Z","timestamp":1646461498000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.74.3.1745-1750.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1128\/IAI.74.3.1745-1750.2006"],"URL":"https:\/\/doi.org\/10.1128\/iai.74.3.1745-1750.2006","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]},"assertion":[{"value":"2005-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}