{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:16:25Z","timestamp":1790381785286,"version":"4.1.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Coccidioides posadasii<\/jats:italic>\n            and\n            <jats:italic>Coccidioides immitis<\/jats:italic>\n            are dimorphic, soil-dwelling pathogenic ascomycetes endemic to the southwestern United States. Infection can result from inhalation of a very few arthroconidia, but following natural infection, long-lived immunity is the norm. Previous work in the field has shown that spherule-derived vaccines afford more protection than those from mycelia. We have used two-dimensional differential in-gel electrophoresis coupled with nano-high-performance liquid chromatography-tandem mass spectrometry to directly assess both absolute abundance and differential expression of proteins in the spherule and the mycelial phases of\n            <jats:italic>C. posadasii<\/jats:italic>\n            with the intent to identify potential vaccine candidates. Peptides derived from 40 protein spots were analyzed and a probable identity was assigned to each. One spherule-abundant protein, identified as Pmp1, showed homology to allergens from\n            <jats:italic>Aspergillus fumigatus<\/jats:italic>\n            and other fungi, all of which exhibit similarity to yeast thiol peroxidases. Recombinant Pmp1 was reactive with serum from individuals with both acute and protracted disease, and evoked protection in two murine models of infection with\n            <jats:italic>C. posadasii<\/jats:italic>\n            . These results demonstrate the utility of proteomic analysis as a point of discovery for protective antigens for possible inclusion in a vaccine candidate to prevent coccidioidomycosis.\n          <\/jats:p>","DOI":"10.1128\/iai.74.3.1865-1872.2006","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T00:18:14Z","timestamp":1140653894000},"page":"1865-1872","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Protein Expression Profiling of\n            <i>Coccidioides posadasii<\/i>\n            by Two-Dimensional Differential In-Gel Electrophoresis and Evaluation of a Newly Recognized Peroxisomal Matrix Protein as a Recombinant Vaccine Candidate"],"prefix":"10.1128","volume":"74","author":[{"given":"Kris I.","family":"Orsborn","sequence":"first","affiliation":[{"name":"Valley Fever Center for Excellence"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa F.","family":"Shubitz","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Peng","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen M.","family":"Kellner","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence"},{"name":"Department of Plant Sciences, College of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc J.","family":"Orbach","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence"},{"name":"Department of Plant Sciences, College of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Haynes","sequence":"additional","affiliation":[{"name":"Bio5 Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John N.","family":"Galgiani","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence"},{"name":"Department of Internal Medicine, College of Medicine, University of Arizona, Tucson, Arizona"},{"name":"Medical and Research Services, Southern Arizona VA Healthcare System, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.2935-2940.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219880000641"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_5_2","first-page":"1039","volume":"9","year":"2002","unstructured":"Ampel, N. M., L. A. Kramer, L. Li, D. S. Carroll, K. M. Kerekes, S. M. Johnson, and D. Pappagianis. 2002. In vitro whole-blood analysis of cellular immunity in patients with active coccidioidomycosis by using the antigen preparation T27K. Clin. Diagn. Lab Immunol.9:1039-1043.","journal-title":"Clin. Diagn. Lab Immunol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401103"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780410001687349"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.78.2.231-239.1959"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.72.6.784-792.1956"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.2.440"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.804-839.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000023889"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/1369378031000156890"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3010-3019.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0086"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.63.1871"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1179\/135100003225002862"},{"key":"e_1_3_2_18_2","first-page":"153","volume":"159","year":"1993","unstructured":"Galgiani, J. N. 1993. Coccidioidomycosis. West. J. Med.159:153-171.","journal-title":"West. J. Med."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/313747"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.7.2587-2595.1995"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(99)00496-5"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.1720"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.6.9702087"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1530"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00442634"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.999-1005.2005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(03)00167-7"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.5848-5853.1999"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3539-3545.2002"},{"key":"e_1_3_2_30_2","unstructured":"Kellner E. M. M. A. Mandel J. N. Galgiani and M. J. Orbach. 2005. Serial analysis of gene expression in Coccidioides posadasii abstr. 171. Abstr. 23rd Fungal Genet. Conf."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.424-431.1998"},{"key":"e_1_3_2_32_2","first-page":"198","volume":"12","year":"1997","unstructured":"Klein, B. S. 1997. Role of cell surface molecules of Blastomyces dermatitidis in the pathogenesis and immunobiology of blastomycosis. Semin. Respir. Infect.12:198-205.","journal-title":"Semin. Respir. Infect."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","unstructured":"Knudsen G. M. K. F. Medzihradszky K. C. Lim E. Hansell and J. H. McKerrow. 2005. Proteomic analysis of Schistosoma mansoni cercarial secretions. Mol. Cell Proteomics 4 : 1862-1875.","DOI":"10.1074\/mcp.M500097-MCP200"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362176385190231"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.2878-2887.2001"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.1.487"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M100004-MCP200"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg087"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401136"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.03921.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(02)00209-7"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(02)00280-1"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.3.3.247"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.8.5723"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1080\/15216540252774748"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.105220"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.3287-3289.2002"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362177685190261"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.25915-0"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5654-5661.2004"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04771.x"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3279-3289.1998"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.3.529-534.1983"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.5.1273"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/379466a0"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00104a020"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2342-2345.1998"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.3.1865-1872.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.3.1865-1872.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:24:10Z","timestamp":1646461450000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.74.3.1865-1872.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":56,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1128\/IAI.74.3.1865-1872.2006"],"URL":"https:\/\/doi.org\/10.1128\/iai.74.3.1865-1872.2006","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]},"assertion":[{"value":"2005-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}