{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:26:20Z","timestamp":1767961580461,"version":"3.49.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Borrelia hermsii<\/jats:italic>, the primary etiological agent of tick-borne relapsing fever in North America, binds the complement regulatory protein factor H (FH) as a means of evading opsonophagocytosis and the alternative complement pathway. The ability of FH-binding protein A (FhbA) to bind FH-like protein 1 (FHL-1) has not been assessed previously. In this study, using a whole-cell absorption assay, we demonstrated that<jats:italic>B. hermsii<\/jats:italic>absorbs both FH and FHL-1 from human serum. Consistent with this, affinity ligand binding immunoblot analyses revealed that FH constructs spanning short consensus repeats 1 to 7 and 16 to 20 bind to FhbA. To investigate the molecular basis of the interaction of FhbA with FH\/FHL-1, recombinant FhbA truncated proteins were generated and tested for FH\/FHL-1 binding. Binding required determinants located in both the N- and C-terminal domains of FhbA, suggesting that long-range intramolecular interactions are involved in the formation and presentation of the FH\/FHL-1-binding pocket. To identify specific FhbA residues involved in binding, random mutagenesis was performed. These analyses identified a loop region of FhbA that may serve as a contact point for FH\/FHL-1. The data presented here expand our understanding of the pathogenic mechanisms of the relapsing fever spirochetes and of the molecular nature of the interaction between FH\/FHL-1 and FhbA.<\/jats:p>","DOI":"10.1128\/iai.74.4.2007-2014.2006","type":"journal-article","created":{"date-parts":[[2006,3,21]],"date-time":"2006-03-21T23:12:50Z","timestamp":1142982770000},"page":"2007-2014","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Molecular Analyses of the Interaction of<i>Borrelia hermsii<\/i>FhbA with the Complement Regulatory Proteins Factor H and Factor H-Like Protein 1"],"prefix":"10.1128","volume":"74","author":[{"given":"Kelley M.","family":"Hovis","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice P.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania","family":"Sadlon","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, South Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gauri","family":"Raval","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, South Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard T.","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"},{"name":"Center for the Study of Biological Complexity, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298-0678"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.6195"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.001103"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02132.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.390-397.1991"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.50.4.381-400.1986"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.156.5.1312"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.815-819.1998"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.6.3029"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.2.1191"},{"key":"e_1_3_1_11_2","first-page":"542","volume":"69","year":"2003","unstructured":"Cutler, S., and A. 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