{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:22:14Z","timestamp":1792624934170,"version":"4.5.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            is a major cause of nosocomial infections worldwide, and the rate of resistance to clinically relevant antibiotics, such as methicillin, is increasing; furthermore, there has been an increase in the number of methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            community-acquired infections. Effective treatment and prevention strategies are urgently needed. We investigated the potential of the\n            <jats:italic>S. aureus<\/jats:italic>\n            surface protein iron surface determinant B (IsdB) as a prophylactic vaccine against\n            <jats:italic>S. aureus<\/jats:italic>\n            infection. IsdB is an iron-sequestering protein that is conserved in diverse\n            <jats:italic>S. aureus<\/jats:italic>\n            clinical isolates, both methicillin resistant and methicillin sensitive, and it is expressed on the surface of all isolates tested. The vaccine was highly immunogenic in mice when it was formulated with amorphous aluminum hydroxyphosphate sulfate adjuvant, and the resulting antibody responses were associated with reproducible and significant protection in animal models of infection. The specificity of the protective immune responses in mice was demonstrated by using an\n            <jats:italic>S. aureus<\/jats:italic>\n            strain deficient for IsdB and HarA, a protein with a high level of identity to IsdB. We also demonstrated that IsdB is highly immunogenic in rhesus macaques, inducing a more-than-fivefold increase in antibody titers after a single immunization. Based on the data presented here, IsdB has excellent prospects for use as a vaccine against\n            <jats:italic>S. aureus<\/jats:italic>\n            disease in humans.\n          <\/jats:p>","DOI":"10.1128\/iai.74.4.2215-2223.2006","type":"journal-article","created":{"date-parts":[[2006,3,21]],"date-time":"2006-03-21T23:12:50Z","timestamp":1142982770000},"page":"2215-2223","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":221,"title":["A Novel\n            <i>Staphylococcus aureus<\/i>\n            Vaccine: Iron Surface Determinant B Induces Rapid Antibody Responses in Rhesus Macaques and Specific Increased Survival in a Murine\n            <i>S. aureus<\/i>\n            Sepsis Model"],"prefix":"10.1128","volume":"74","author":[{"given":"Nelly A.","family":"Kuklin","sequence":"first","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desmond J.","family":"Clark","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Secore","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Cook","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie D.","family":"Cope","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessie","family":"McNeely","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane","family":"Noble","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha J.","family":"Brown","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie K.","family":"Zorman","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Pancari","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Fan","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Isett","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce","family":"Burgess","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine","family":"Bryan","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Brownlow","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh","family":"George","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Meinz","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Liddell","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemarie","family":"Kelly","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loren","family":"Schultz","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Montgomery","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Onishi","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Losada","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Martin","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Ebert","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Y.","family":"Tan","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy L.","family":"Schofield","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"Nagy","sequence":"additional","affiliation":[{"name":"Intercell AG, Vienna Biocenter 6, A-1030 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Meineke","sequence":"additional","affiliation":[{"name":"Intercell AG, Vienna Biocenter 6, A-1030 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph G.","family":"Joyce","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myra B.","family":"Kurtz","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Caulfield","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin U.","family":"Jansen","sequence":"additional","affiliation":[{"name":"Vaxgen Inc., 1000 Marina Boulevard, Brisbane, California 94005"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"McClements","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annaliesa S.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Merck and Co. Inc., 440 Sumneytown Pike, WP16 100, West Point, Pennsylvania 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.02.019"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-9-reports0047"},{"key":"e_1_3_1_4_2","first-page":"127","volume":"63","year":"1986","unstructured":"Barrett, D. J., and E. M. Ayoub. 1986. IgG2 subclass restriction of antibody to pneumococcal polysaccharides. Clin. Exp. Immunol.63:127-134.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_1_5_2","first-page":"305","volume":"1","year":"1984","unstructured":"Briles, D. E., C. Forman, S. Hudak, and J. L. Claflin. 1984. The effects of subclass on the ability of anti-phosphocholine antibodies to protect mice from fatal infection with Streptococcus pneumoniae. J. Mol. Cell. Immunol.1:305-309.","journal-title":"J. Mol. Cell. Immunol."},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1450-1456.2000"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03542.x"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1008-1015.2000"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092569199"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.06.043"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6864-6870.2003"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/40.1.135"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/377308"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)00103-8"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/324430"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2740-2748.2003"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/713608018"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00033-0"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808203390806"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3389-3395.2002"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1994.tb00010.x"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1081147"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032523999"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.5.1839-1841.1996"},{"key":"e_1_3_1_25_2","first-page":"902","volume":"51","year":"2002","unstructured":"Miller, D., V. Urdaneta, and S. Park. 2002. Vancomycin-resistant Staphylococcus aureus\u2014Pennsylvania, 2002. Morb. Mortal. Wkly. Rep.51:902.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/323459"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4430"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1823"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/315023"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.218-234.2004"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2004.03.007"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.08.015"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.96.3.600"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1086\/516520"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1993.1073"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa011297"},{"key":"e_1_3_1_37_2","first-page":"565","volume":"51","year":"2002","unstructured":"Sievert, D. M., M. L. Boulton, G. Stoltman, D. Johnson, M. G. Stobierski, F. P. Downes, P. A. Somsel, J. T. Rudrik, W. Brown, W. Hafeez, T. Lundstrom, E. Flanagan, R. Johnson, J. Mitchell, and S. Chang. 2002. Staphylococcus aureus resistant to vancomycin\u2014United States, 2002. Morb. Mortal. Wkly. Rep.51:565-567.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2003.12.008"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1067\/mic.2002.130032"},{"key":"e_1_3_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2003.09.002"},{"key":"e_1_3_1_41_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.103001.114744"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.4.2215-2223.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.74.4.2215-2223.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:24:44Z","timestamp":1646461484000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.74.4.2215-2223.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1128\/IAI.74.4.2215-2223.2006"],"URL":"https:\/\/doi.org\/10.1128\/iai.74.4.2215-2223.2006","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4]]},"assertion":[{"value":"2005-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}