{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T05:58:58Z","timestamp":1697090338814},"reference-count":81,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            (gonococcus [GC]), is highly adapted to the human host, the only known reservoir for gonococcal infection. However, since it is sexually transmitted, infection of a new host likely requires a regulatory response on the part of the gonococcus to respond to this significant change in environment. We previously showed that adherence of gonococci to epithelial cells results in changes of gene expression in the bacteria that presumably prepare them for subsequent steps in the infection process. Expression of the heat shock sigma factor gene,\n            <jats:italic>rpoH<\/jats:italic>\n            , was shown to be important for the invasion step, as gonococci depleted for\n            <jats:italic>rpoH<\/jats:italic>\n            were reduced in their ability to invade epithelial cells. Here, we show that of the genes induced in adherent gonococci, two are part of the gonococcal RpoH regulon. When RpoH is depleted, expression of these genes is no longer induced by host cell contact, indicating that RpoH is mediating the host cell induction response of these genes. One RpoH-dependent gene, NGO0376, is shown to be important for invasion of epithelial cells, consistent with earlier observations that RpoH is necessary for this step of infection. Two genes, NGO1684 and NGO0340, while greatly induced by host cell contact, were found to be RpoH independent, indicating that more than one regulator is involved in the response to host cell contact. Furthermore, NGO0340, but not NGO1684, was shown to be important for both adherence and invasion of epithelial cells, suggesting a complex regulatory network in the response of gonococci to contact with host cells.\n          <\/jats:p>","DOI":"10.1128\/iai.74.5.2767-2776.2006","type":"journal-article","created":{"date-parts":[[2006,4,18]],"date-time":"2006-04-18T22:15:01Z","timestamp":1145398501000},"page":"2767-2776","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["RpoH Mediates the Expression of Some, but Not All, Genes Induced in\n            <i>Neisseria gonorrhoeae<\/i>\n            Adherent to Epithelial Cells"],"prefix":"10.1128","volume":"74","author":[{"given":"Ying","family":"Du","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics and Center for Microbial Pathogenesis, Michigan State University, East Lansing, Michigan 48824-1101"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy Grove","family":"Arvidson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics and Center for Microbial Pathogenesis, Michigan State University, East Lansing, Michigan 48824-1101"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.5.2660-2666.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00206-3"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00112.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1999.tb01317.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01664.x"},{"key":"e_1_3_2_7_2","unstructured":"Neisseria gonorrhoeae annotated genome sequence database 2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-50"},{"key":"e_1_3_2_9_2","first-page":"155","year":"1978","unstructured":"Brinton, C. 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