{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:23:33Z","timestamp":1792185813627,"version":"4.4.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel putative autotransporter protein (NMB1998) was identified in the available genomic sequence of meningococcal strain MC58 (ET-5; ST-32). The\n            <jats:italic>mspA<\/jats:italic>\n            gene is absent from the genomic sequences of meningococcal strain Z2491 (ET-IV; ST-4) and the gonococcal strain FA1090. An orthologue is present in the meningococcal strain FAM18 (ET-37; ST-11), but the sequence contains a premature stop codon, suggesting that the protein may not be expressed in this strain. MspA is predicted to be a 157-kDa protein with low cysteine content, and it exhibits 36 and 33% identity to the meningococcal autotransporter proteins immunoglobulin A1 (IgA1) protease and App, respectively. Search of the Pfam database predicts the presence of IgA1 protease and autotransporter \u03b2-barrel domains. MspA was cloned, and a recombinant protein of the expected size was expressed and after being affinity purified was used to raise rabbit polyclonal monospecific antiserum. Immunoblot studies showed that ca. 125- and 95-kDa fragments of MspA are secreted in meningococcal strain MC58, which are absent from the isogenic mutant. Secretion of MspA was shown to be modified in an AspA isogenic mutant. A strain survey showed that MspA is expressed by all ST-32 and ST-41\/44 (lineage 3) strains, but none of the ST-8 (A4) strains examined. Sera from patients convalescing from meningococcal disease were shown to contain MspA-specific antibodies. In bactericidal assays, anti-MspA serum was shown to kill the homologous strain (MC58) and another ST-32 strain.\n            <jats:italic>Escherichia coli<\/jats:italic>\n            -expressing recombinant MspA was shown to adhere to both human bronchial epithelial cells and brain microvascular endothelial cells.\n          <\/jats:p>","DOI":"10.1128\/iai.74.5.2957-2964.2006","type":"journal-article","created":{"date-parts":[[2006,4,18]],"date-time":"2006-04-18T22:15:01Z","timestamp":1145398501000},"page":"2957-2964","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":79,"title":["Characterization of MspA, an Immunogenic Autotransporter Protein That Mediates Adhesion to Epithelial and Endothelial Cells in\n            <i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"74","author":[{"given":"D. P. J.","family":"Turner","sequence":"first","affiliation":[{"name":"Molecular Biology and Immunology Group, Institute of Infections, Immunity and Inflammation, School of Molecular Medical Sciences, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. G.","family":"Marietou","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Group, Institute of Infections, Immunity and Inflammation, School of Molecular Medical Sciences, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Johnston","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Group, Institute of Infections, Immunity and Inflammation, School of Molecular Medical Sciences, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. K. L.","family":"Ho","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Group, Institute of Infections, Immunity and Inflammation, School of Molecular Medical Sciences, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Group, Institute of Infections, Immunity and Inflammation, School of Molecular Medical Sciences, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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