{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:35:43Z","timestamp":1753882543101,"version":"3.41.2"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI088075"],"award-info":[{"award-number":["AI088075"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI153197"],"award-info":[{"award-number":["AI153197"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI174646"],"award-info":[{"award-number":["AI174646"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,3,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Cytokinins are adenine-based hormones that have been well-characterized in plants but are also made by bacteria, including the human-exclusive pathogen\n              <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n              . Like plants,\n              <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n              uses cytokinins to regulate gene expression. We previously established that cytokinin overaccumulation in\n              <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n              results in a buildup of aldehydes produced during cytokinin breakdown. In plants, dedicated enzymes called cytokinin oxidases convert cytokinins into adenine and various aldehydes. Proteasome degradation-deficient\n              <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n              , which cannot degrade the cytokinin-producing enzyme Log, accumulates several cytokinins and at least one cytokinin-associated aldehyde, resulting in increased sensitivity to nitric oxide and copper. We therefore hypothesized that\n              <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n              encodes one or more cytokinin oxidases, and disruption of this enzyme might restore resistance to nitric oxide and copper in a proteasome-defective strain. Using a homology-based search, we identified Rv3719 as a protein with high similarity to a plant cytokinin oxidase. Deletion of this gene, however, did not restore nitric oxide or copper resistance to a degradation-defective mutant. Instead, we observed increased copper sensitivity when Rv3719 was deleted from either wild-type or proteasome-defective strains. Finally, we characterized Rv3718c, a protein encoded adjacent to Rv3719, and found that it bound a cytokinin with high specificity. Collectively, these data support a role for cytokinin activity in\n              <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n              physiology that remains to be further elucidated.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>Numerous bacterial species encode cytokinin-producing enzymes, the functions of which are almost completely unknown. This work contributes new knowledge to the cytokinin field for bacteria and reveals further conservation of cytokinin-associated proteins between plants and prokaryotes.<\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jb.00003-25","type":"journal-article","created":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T14:01:41Z","timestamp":1740664901000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of a cytokinin-binding protein locus in\n            <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","volume":"207","author":[{"given":"Jin Hee","family":"Yoo","sequence":"first","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine","place":["New York, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Santarossa","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, New York University School of Medicine","place":["New York, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine","place":["New York, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damian","family":"Ekiert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine","place":["New York, USA"]},{"name":"Department of Cell Biology, New York University School of Medicine","place":["New York, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5043-7548","authenticated-orcid":true,"given":"K. Heran","family":"Darwin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine","place":["New York, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_3_2_2","DOI":"10.1126\/science.1091176"},{"doi-asserted-by":"publisher","key":"e_1_3_3_3_2","DOI":"10.1038\/nm1683"},{"doi-asserted-by":"publisher","key":"e_1_3_3_4_2","DOI":"10.1128\/MMBR.00036-16"},{"doi-asserted-by":"publisher","key":"e_1_3_3_5_2","DOI":"10.1016\/j.molcel.2015.01.024"},{"doi-asserted-by":"publisher","key":"e_1_3_3_6_2","DOI":"10.1128\/mbio.00363-23:e0036323"},{"doi-asserted-by":"publisher","key":"e_1_3_3_7_2","DOI":"10.1146\/annurev.arplant.57.032905.105231"},{"doi-asserted-by":"publisher","key":"e_1_3_3_8_2","DOI":"10.1105\/tpc.109.068676"},{"doi-asserted-by":"publisher","key":"e_1_3_3_9_2","DOI":"10.1016\/j.tplants.2007.11.005"},{"doi-asserted-by":"publisher","key":"e_1_3_3_10_2","DOI":"10.1128\/mBio.00989-18"},{"doi-asserted-by":"publisher","key":"e_1_3_3_11_2","DOI":"10.1042\/BJ20060280"},{"doi-asserted-by":"publisher","key":"e_1_3_3_12_2","DOI":"10.1016\/S0022-2836(05)80360-2"},{"doi-asserted-by":"publisher","key":"e_1_3_3_13_2","DOI":"10.1385\/1-59259-890-0:571"},{"doi-asserted-by":"publisher","key":"e_1_3_3_14_2","DOI":"10.5935\/0305-7518.20010047"},{"doi-asserted-by":"publisher","key":"e_1_3_3_15_2","DOI":"10.1038\/s41586-021-03819-2"},{"doi-asserted-by":"publisher","key":"e_1_3_3_16_2","DOI":"10.1111\/j.1365-2958.2010.07431.x"},{"doi-asserted-by":"publisher","key":"e_1_3_3_17_2","DOI":"10.1073\/pnas.2134250100"},{"doi-asserted-by":"publisher","key":"e_1_3_3_18_2","DOI":"10.1099\/00221287-148-10-3007"},{"key":"e_1_3_3_19_2","volume-title":"Molecular genetics of mycobacteria","author":"Hatfull GF","year":"2000","unstructured":"Hatfull GF, Jacobs WRJ. 2000. Molecular genetics of mycobacteria. ASM Press, Washington, DC."},{"doi-asserted-by":"publisher","key":"e_1_3_3_20_2","DOI":"10.1038\/nmeth.2019"},{"doi-asserted-by":"publisher","key":"e_1_3_3_21_2","DOI":"10.1128\/mBio.00876-13"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.00003-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.00003-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T22:12:37Z","timestamp":1748297557000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.00003-25"}},"subtitle":[],"editor":[{"given":"Patricia A.","family":"Champion","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,20]]}},"alternative-id":["10.1128\/jb.00003-25"],"URL":"https:\/\/doi.org\/10.1128\/jb.00003-25","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"type":"print","value":"0021-9193"},{"type":"electronic","value":"1098-5530"}],"subject":[],"published":{"date-parts":[[2025,3,20]]},"assertion":[{"value":"2025-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00003-25"}}