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Each cp32 carries a locus encoding one or two different Erp outer membrane, surface-exposed lipoproteins. Many of the Erp proteins bind a host complement regulator, factor H, which is hypothesized to protect the spirochete from complement-mediated killing. We now describe the isolation and characterization of a novel, chromosomally encoded protein, EbfC, that binds specific DNA sequences located immediately 5\u2032 of all<jats:italic>erp<\/jats:italic>loci. This is one of the first site-specific DNA-binding proteins to be identified in any spirochete. The location of the<jats:italic>ebfC<\/jats:italic>gene on the<jats:italic>B. burgdorferi<\/jats:italic>chromosome suggests that the cp32 prophages have evolved to use this bacterial host protein for their own benefit and that EbfC probably plays additional roles in the bacterium. A wide range of other bacteria encode homologs of EbfC, none of which have been well characterized, so demonstration that<jats:italic>B. burgdorferi<\/jats:italic>EbfC is a site-specific DNA-binding protein has broad implications across the eubacterial kingdom.<\/jats:p>","DOI":"10.1128\/jb.00005-06","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T21:31:37Z","timestamp":1149197497000},"page":"4331-4339","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["<i>Borrelia burgdorferi<\/i>EbfC, a Novel, Chromosomally Encoded Protein, Binds Specific DNA Sequences Adjacent to<i>erp<\/i>Loci on the Spirochete's Resident cp32 Prophages"],"prefix":"10.1128","volume":"188","author":[{"given":"Kelly","family":"Babb","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Bykowski","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P.","family":"Riley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Clarke","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kentucky, Lexington, Kentucky 40536-0055"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"DeMoll","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298"},{"name":"Department of Chemistry, University of Kentucky, Lexington, Kentucky 40536-0055"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1073\/pnas.012602299","volume":"99","year":"2002","unstructured":"Akerley, B. 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