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In order to identify factors that control the production, activity, or secretion of this essential virulence factor, we constructed a\n                    <jats:italic>Himar1 mariner<\/jats:italic>\n                    transposon delivery system and screened 50,000 mutants for a hypohemolytic phenotype on blood agar plates. Approximately 200 hypohemolytic mutants were identified, and the 51 most prominent mutants were screened ex vivo for intracellular growth defects. Eight mutants with a phenotype were identified, and they contained insertions in the following genes: lmo0964 (similar to\n                    <jats:italic>yjbH<\/jats:italic>\n                    ), lmo1268 (\n                    <jats:italic>clpX<\/jats:italic>\n                    ), lmo1401 (similar to\n                    <jats:italic>ymdB<\/jats:italic>\n                    ), lmo1575 (similar to\n                    <jats:italic>ytqI<\/jats:italic>\n                    ), lmo1695 (\n                    <jats:italic>mprF<\/jats:italic>\n                    ), lmo1821 (similar to\n                    <jats:italic>prpC<\/jats:italic>\n                    ), lmo2219 (\n                    <jats:italic>prsA2<\/jats:italic>\n                    ), and lmo2460 (similar to\n                    <jats:italic>cggR<\/jats:italic>\n                    ). Some of these genes are involved in previously unexplored areas of research with\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    : the genes\n                    <jats:italic>yjbH<\/jats:italic>\n                    and\n                    <jats:italic>clpX<\/jats:italic>\n                    regulate the disulfide stress response in\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    , and the\n                    <jats:italic>prpC<\/jats:italic>\n                    phosphatase has been implicated in virulence in other gram-positive pathogens. Here we demonstrate that\n                    <jats:italic>prsA2<\/jats:italic>\n                    , an extracytoplasmic peptidyl-prolyl\n                    <jats:italic>cis<\/jats:italic>\n                    \/\n                    <jats:italic>trans<\/jats:italic>\n                    isomerase, is critical for virulence and contributes to the folding of LLO and to the activity of another virulence factor, the broad-range phospholipase C (PC-PLC). Furthermore, although it has been shown that\n                    <jats:italic>prsA2<\/jats:italic>\n                    expression is linked to PrfA, the master virulence transcription factor in\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    pathogenesis, we demonstrate that\n                    <jats:italic>prsA2<\/jats:italic>\n                    is not directly controlled by PrfA. Finally, we show that PrsA2 is involved in flagellum-based motility, indicating that this factor likely serves a broad physiological role.\n                  <\/jats:p>","DOI":"10.1128\/jb.00016-09","type":"journal-article","created":{"date-parts":[[2009,4,17]],"date-time":"2009-04-17T21:07:23Z","timestamp":1240002443000},"page":"3950-3964","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":110,"title":["Development of a\n                    <i>mariner<\/i>\n                    -Based Transposon and Identification of\n                    <i>Listeria monocytogenes<\/i>\n                    Determinants, Including the Peptidyl-Prolyl Isomerase PrsA2, That Contribute to Its Hemolytic Phenotype"],"prefix":"10.1128","volume":"191","author":[{"given":"Jason","family":"Zemansky","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin C.","family":"Kline","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua J.","family":"Woodward","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jess H.","family":"Leber","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u0301le\u0300ne","family":"Marquis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology"},{"name":"School of Public Health, University of California, Berkeley, California 94720-3202"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1","volume":"257","year":"2001","unstructured":"Alouf, J. 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