{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:20:37Z","timestamp":1791984037766,"version":"4.4.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,6,15]],"date-time":"2012-06-15T00:00:00Z","timestamp":1339718400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Outer membrane vesicles (OMVs) have been identified in a wide range of bacteria, yet little is known of their biogenesis. It has been proposed that OMVs can act as long-range toxin delivery vectors and as a novel stress response. We have found that the formation of OMVs in the Gram-negative opportunistic pathogen\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Serratia marcescens<\/jats:named-content>\n            is thermoregulated, with a significant amount of OMVs produced at 22 or 30\u00b0C and negligible quantities formed at 37\u00b0C under laboratory conditions. Inactivation of the synthesis of the enterobacterial common antigen (ECA) resulted in a hypervesiculation phenotype, supporting the hypothesis that OMVs are produced in response to stress. We demonstrate that the phenotype can be reversed to wild-type (WT) levels upon the loss of the Rcs phosphorelay response regulator RcsB, but not RcsA, suggesting a role for the Rcs phosphorelay in the production of OMVs. MS fingerprinting of the OMVs provided evidence of cargo selection within wild-type cells, suggesting a possible role for\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Serratia<\/jats:named-content>\n            OMVs in toxin delivery. In addition, OMV-associated cargo proved toxic upon injection into the haemocoel of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Galleria mellonella<\/jats:named-content>\n            larvae. These experiments demonstrate that OMVs are the result of a regulated process in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Serratia<\/jats:named-content>\n            and suggest that OMVs could play a role in virulence.\n          <\/jats:p>","DOI":"10.1128\/jb.00016-12","type":"journal-article","created":{"date-parts":[[2012,4,8]],"date-time":"2012-04-08T04:48:27Z","timestamp":1333860507000},"page":"3241-3249","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":131,"title":["Biogenesis of Outer Membrane Vesicles in Serratia marcescens Is Thermoregulated and Can Be Induced by Activation of the Rcs Phosphorelay System"],"prefix":"10.1128","volume":"194","author":[{"given":"Kenneth J.","family":"McMahon","sequence":"first","affiliation":[{"name":"Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Castelli","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Departamento de Microbiolog\u00eda, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Suipacha, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora Garc\u00eda","family":"Vescovi","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Departamento de Microbiolog\u00eda, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Suipacha, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario F.","family":"Feldman","sequence":"additional","affiliation":[{"name":"Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1099\/mic.0.2007\/012427-0","article-title":"The role of the Serratia marcescens SdeAB multidrug efflux pump and TolC homologue in fluoroquinolone resistance studied via gene-knockout mutagenesis","volume":"154","author":"Begic S","year":"2008","unstructured":"BegicS WorobecEA. 2008. 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