{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:03:39Z","timestamp":1789189419155,"version":"build-2803163510"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,6,15]],"date-time":"2011-06-15T00:00:00Z","timestamp":1308096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2011,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previous studies showed that deletion of genes c3405 to c3410 from PAI-\n            <jats:italic>metV<\/jats:italic>\n            , a genomic island from\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            CFT073, results in a strain that fails to compete with wild-type CFT073 after a transurethral cochallenge in mice and is deficient in the ability to independently colonize the mouse kidney. Our analysis of c3405 to c3410 suggests that these genes constitute an operon with a role in the internalization and utilization of an unknown carbohydrate. This operon is not found in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            K-12 but is present in a small number of pathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Shigella boydii<\/jats:named-content>\n            strains. One of the genes, c3406, encodes a protein with significant homology to the sugar isomerase domain of arabinose 5-phosphate isomerases but lacking the tandem cystathionine beta-synthase domains found in the other arabinose 5-phosphate isomerases of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            . We prepared recombinant c3406 protein, found it to possess arabinose 5-phosphate isomerase activity, and characterized this activity in detail. We also constructed a c3406 deletion mutant of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            CFT073 and demonstrated that this deletion mutant was still able to compete with wild-type CFT073 in a transurethral cochallenge in mice and could colonize the mouse kidney. These results demonstrate that the presence of c3406 is not essential for a pathogenic phenotype.\n          <\/jats:p>","DOI":"10.1128\/jb.00033-11","type":"journal-article","created":{"date-parts":[[2011,4,16]],"date-time":"2011-04-16T07:31:37Z","timestamp":1302939097000},"page":"2981-2988","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["A Unique Arabinose 5-Phosphate Isomerase Found within a Genomic Island Associated with the Uropathogenicity of Escherichia coli CFT073"],"prefix":"10.1128","volume":"193","author":[{"given":"Joshua A.","family":"Mosberg","sequence":"first","affiliation":[{"name":"Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Yep","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy C.","family":"Meredith","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065"},{"name":"Present address: Novartis Institute for Biomedical Research, Cambridge, MA 02139."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-Fen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tod P.","family":"Holler","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry L. 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