{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:17:38Z","timestamp":1790745458564,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T00:00:00Z","timestamp":1529020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Fund for Tick-Borne Diseases"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2018,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The SpoVG protein of\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            , the Lyme disease spirochete, binds to specific sites of DNA and RNA. The bacterium regulates transcription of\n            <jats:italic>spoVG<\/jats:italic>\n            during the natural tick-mammal infectious cycle and in response to some changes in culture conditions. Bacterial levels of\n            <jats:italic>spoVG<\/jats:italic>\n            mRNA and SpoVG protein did not necessarily correlate, suggesting that posttranscriptional mechanisms also control protein levels. Consistent with this, SpoVG binds to its own mRNA, adjacent to the ribosome-binding site. SpoVG also binds to two DNA sites in the\n            <jats:italic>glpFKD<\/jats:italic>\n            operon and to two RNA sites in\n            <jats:italic>glpFKD<\/jats:italic>\n            mRNA; that operon encodes genes necessary for glycerol catabolism and is important for colonization in ticks. In addition, spirochetes engineered to dysregulate\n            <jats:italic>spoVG<\/jats:italic>\n            exhibited physiological alterations.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            persists in nature by cycling between ticks and vertebrates. Little is known about how the bacterium senses and adapts to each niche of the cycle. The present studies indicate that\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            controls production of SpoVG and that this protein binds to specific sites of DNA and RNA in the genome and transcriptome, respectively. Altered expression of\n            <jats:italic>spoVG<\/jats:italic>\n            exerts effects on bacterial replication and other aspects of the spirochete's physiology.\n          <\/jats:p>","DOI":"10.1128\/jb.00033-18","type":"journal-article","created":{"date-parts":[[2018,4,5]],"date-time":"2018-04-05T10:49:14Z","timestamp":1522925354000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Borrelia burgdorferi SpoVG DNA- and RNA-Binding Protein Modulates the Physiology of the Lyme Disease Spirochete"],"prefix":"10.1128","volume":"200","author":[{"given":"Christina R.","family":"Savage","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon L.","family":"Jutras","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Bestor","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kit","family":"Tilly","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Tourand","sequence":"additional","affiliation":[{"name":"School of Medicine and Health Science, University of North Dakota, Grand Forks, North Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip E.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Brissette","sequence":"additional","affiliation":[{"name":"School of Medicine and Health Science, University of North Dakota, Grand Forks, North Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"},{"name":"Department of Entomology, University of Kentucky, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"167","article-title":"Ticks and Borrelia: model systems for investigating pathogen-arthropod interactions","volume":"5","author":"Schwan TG","year":"1996","unstructured":"Schwan TG. 1996. Ticks and Borrelia: model systems for investigating pathogen-arthropod interactions. Infect Agents Dis 5:167\u2013181.","journal-title":"Infect Agents Dis"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_2_4_2","volume-title":"Spirochete biology: the post genomic era","author":"Stevenson B","unstructured":"Stevenson B, Seshu J. Regulation of gene and protein expression in the Lyme disease spirochete. In Adler B (ed), Spirochete biology: the post genomic era, in press. Springer-Nature, Heidelberg, Germany."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.148.1.341-351.1981"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066683"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00240-16"},{"key":"e_1_3_2_8_2","first-page":"248","volume-title":"Morphology, physiology, and behavioral biology of ticks","author":"Coons LB","year":"1986","unstructured":"Coons LB, Rosell-Davis R, Tarnowski BI. 1986. Bloodmeal digestion in ticks, p 248\u2013279. In Sauer JR, Hair JA (ed), Morphology, physiology, and behavioral biology of ticks. Ellis Horwood Ltd., New York, NY."},{"key":"e_1_3_2_9_2","volume-title":"Biology of ticks","author":"Sonenshine DE","year":"1991","unstructured":"Sonenshine DE. 1991. Biology of ticks, vol 1. Oxford University Press, New York, NY."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00036-6"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39401"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2187-4"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002102"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118063"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-4-1033"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12882"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1073\/pnas.92.7.2909","article-title":"Induction of an outer surface protein on Borrelia burgdorferi during tick feeding","volume":"92","author":"Schwan TG","year":"1995","unstructured":"Schwan TG, Piesman J, Golde WT, Dolan MC, Rosa PA. 1995. Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc Natl Acad Sci U S A 92:2909\u20132913.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.11.4535-4539.1995"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032667699"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1689-1705.2003"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-006-0690-2"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3398-3"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00530-15"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.9.2745-2756.2004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01956-12"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002133"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005160"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01204-17"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02104.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06621.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.3.957-965.2003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.2.444-452.2003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164165"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1180"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00819-13"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.491357"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00052-14"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-34-4-496"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6943-6952.2003"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2139-2150.2002"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2011.05.004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mc12c01s4","article-title":"Laboratory maintenance of Borrelia burgdorferi","author":"Z\u00fcckert WR","year":"2007","unstructured":"Z\u00fcckert WR. 2007. Laboratory maintenance of Borrelia burgdorferi. Curr Protoc Microbiol Chapter 12:Unit 12C.1.","journal-title":"Curr Protoc Microbiol"},{"key":"e_1_3_2_44_2","article-title":"Example of real-time quantitative reverse transcription-PCR (Q-RT-PCR) analysis of bacterial gene expression during mammalian infection: Borrelia burgdorferi in mouse tissues","author":"Miller JC","year":"2005","unstructured":"Miller JC. 2005. Example of real-time quantitative reverse transcription-PCR (Q-RT-PCR) analysis of bacterial gene expression during mammalian infection: Borrelia burgdorferi in mouse tissues. Curr Protoc Microbiol Chapter 1D:Unit 1D.3.","journal-title":"Curr Protoc Microbiol"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-290"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00714-07"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00758-12"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.47.249"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp027"},{"key":"e_1_3_2_50_2","first-page":"61","volume-title":"Metagenomics and its applications in agriculture, biomedicine and environmental studies","author":"Solovyev V","year":"2011","unstructured":"Solovyev V, Salamov A. 2011. Automatic annotation of microbial genomes and metagenomic sequences, p 61\u201378. In Li RW (ed), Metagenomics and its applications in agriculture, biomedicine and environmental studies. Nova Science Publishers, Hauppauge, NY."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00033-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00033-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:45:04Z","timestamp":1627580704000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00033-18"}},"subtitle":[],"editor":[{"given":"Yves V.","family":"Brun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,6,15]]},"references-count":49,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,6,15]]}},"alternative-id":["10.1128\/JB.00033-18"],"URL":"https:\/\/doi.org\/10.1128\/jb.00033-18","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,15]]},"assertion":[{"value":"2018-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}