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Positive regulatory factor A (PrfA) is a pleiotropic master activator of virulence genes of\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              that becomes active upon the entry of the bacterium into the cytosol of infected cells.\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              can survive and multiply at low temperatures; this is accomplished through the maintenance of appropriate membrane fluidity via branched-chain fatty acid (BCFA) synthesis. Branched-chain \u03b1-keto acid dehydrogenase (BKD), which is composed of four polypeptides encoded by\n              <jats:italic>lpd<\/jats:italic>\n              ,\n              <jats:italic>bkdA1<\/jats:italic>\n              ,\n              <jats:italic>bkdA2<\/jats:italic>\n              , and\n              <jats:italic>bkdB<\/jats:italic>\n              , is known to play a vital role in BCFA biosynthesis. Here, we constructed BKD-deficient\n              <jats:italic>Listeria<\/jats:italic>\n              strains by in-frame deletion of\n              <jats:italic>lpd<\/jats:italic>\n              ,\n              <jats:italic>bkdA1<\/jats:italic>\n              ,\n              <jats:italic>bkdA2<\/jats:italic>\n              , and\n              <jats:italic>bkdB<\/jats:italic>\n              genes. To determine the role in\n              <jats:italic>in vivo<\/jats:italic>\n              and\n              <jats:italic>in vitro<\/jats:italic>\n              , mouse model challenges, plaque assay in murine L2 fibroblast, and intracellular replication in J744A.1 macrophage were conducted. BKD-deficient strains exhibited defects in BCFA composition, virulence, and PrfA-regulon function within the host cells. Transcriptomics analysis revealed that the transcript level of the PrfA-regulon was lower in \u0394\n              <jats:italic>bkdA1<\/jats:italic>\n              strain than those in the wild-type. This study demonstrates that\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              strains lacking BKD complex components were defective in PrfA-regulon function, and full activation of wild-type\n              <jats:italic>prfA<\/jats:italic>\n              may not occur within host cells in the absence of BKD. Further study will investigate the consequences of BKD deletion on PrfA function through altering BCFA catabolism.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Listeria monocytogenes<\/jats:italic>\n                is the causative agent of listeriosis, a disease with a high mortality rate. In this study, we have shown that the deletion of BKD can impact the function of PrfA and the PrfA-regulon. The production of virulence proteins within host cells is necessary for\n                <jats:italic>L. monocytogenes<\/jats:italic>\n                to promote its intracellular survival and is likely dependent on membrane integrity. We thus report a link between\n                <jats:italic>L. monocytogenes<\/jats:italic>\n                membrane integrity and the function of PrfA. This knowledge will increase our understanding of\n                <jats:italic>L. monocytogenes<\/jats:italic>\n                pathogenesis, which may provide insight into the development of antimicrobial agents.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jb.00033-24","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T13:02:11Z","timestamp":1718888531000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["An insight into the role of branched-chain \u03b1-keto acid dehydrogenase (BKD) complex in branched-chain fatty acid biosynthesis and virulence of\n            <i>Listeria monocytogenes<\/i>"],"prefix":"10.1128","volume":"206","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2102-7176","authenticated-orcid":false,"given":"Q M Monzur","family":"Kader Chowdhury","sequence":"first","affiliation":[{"name":"Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamima","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshmi","family":"Narayanan","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seto C.","family":"Ogunleye","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangshang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinh","family":"Thu","sequence":"additional","affiliation":[{"name":"Tyson Foods, R&amp;D Ingredient Solutions, Springdale, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1322-3978","authenticated-orcid":true,"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark L.","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2439-0659","authenticated-orcid":true,"given":"Hossam","family":"Abdelhamed","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","unstructured":"FDA. 2018. Get the Facts about Listeria. Available from: https:\/\/www.fda.gov\/animal-veterinary\/animal-health-literacy\/get-facts-about-listeria. Retrieved 31 Oct 2022."},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00404.x"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00555-18"},{"key":"e_1_3_5_5_2","unstructured":"CDC. 2022. Listeria (Listeriosis) | Listeria. Available from: https:\/\/www.cdc.gov\/listeria\/index.html. 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