{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T18:29:16Z","timestamp":1772389756364,"version":"3.50.1"},"reference-count":84,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI051486"],"award-info":[{"award-number":["R01 AI051486"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20 GM103546"],"award-info":[{"award-number":["P20 GM103546"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The rRNA genes of\n            <jats:named-content content-type=\"genus-species\">Borrelia<\/jats:named-content>\n            (\n            <jats:named-content content-type=\"genus-species\">Borreliella<\/jats:named-content>\n            )\n            <jats:named-content content-type=\"genus-species\">burgdorferi<\/jats:named-content>\n            are unusually organized; the spirochete has a single 16S rRNA gene that is more than 3 kb from a tandem pair of 23S-5S rRNA operons. We generated an\n            <jats:italic>rnc<\/jats:italic>\n            null mutant in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            that exhibits a pleiotropic phenotype, including decreased growth rate and increased cell length. Here, we demonstrate that endoribonuclease III (RNase III) is, as expected, involved in processing the 23S rRNA in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            . The 5\u2032 and 3\u2032 ends of the three rRNAs were determined in the wild type and\n            <jats:italic>rnc<\/jats:italic>\n            <jats:sub>Bb<\/jats:sub>\n            mutants; the results suggest that RNase III in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            is required for the full maturation of the 23S rRNA but not for the 5S rRNA nor, curiously, for the 16S rRNA.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Lyme disease, the most common tick-borne zoonosis in the Northern Hemisphere, is caused by the bacterium\n            <jats:named-content content-type=\"genus-species\">Borrelia<\/jats:named-content>\n            (\n            <jats:named-content content-type=\"genus-species\">Borreliella<\/jats:named-content>\n            )\n            <jats:named-content content-type=\"genus-species\">burgdorferi<\/jats:named-content>\n            , a member of the deeply branching spirochete phylum.\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            carries a limited suite of ribonucleases, enzymes that cleave RNA during processing and degradation. Several ribonucleases, including RNase III, are involved in the production of ribosomes, which catalyze translation and are a major target of antibiotics. This is the first study to dissect the role of an RNase in any spirochete. We demonstrate that an RNase III mutant is viable but has altered processing of rRNA.\n          <\/jats:p>","DOI":"10.1128\/jb.00035-18","type":"journal-article","created":{"date-parts":[[2018,4,9]],"date-time":"2018-04-09T11:08:22Z","timestamp":1523272102000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["RNase III Processing of rRNA in the Lyme Disease Spirochete Borrelia burgdorferi"],"prefix":"10.1128","volume":"200","author":[{"given":"Melissa L.","family":"Anacker","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Drecktrah","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard D.","family":"LeCoultre","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meghan","family":"Lybecker","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana, USA"},{"name":"Department of Biology, University of Colorado, Colorado Springs, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Scott","family":"Samuels","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, Montana, USA"},{"name":"Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081302"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081301"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3603026"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_2_7_2","first-page":"27","volume-title":"Borrelia: molecular biology, host interaction and pathogenesis","author":"Casjens SR","year":"2010","unstructured":"Casjens SR, Eggers CH, Schwartz I. 2010. Borrelia genomics: chromosome, plasmids, bacteriophages and genetic variation, p 27\u201353. In Samuels DS, Radolf JD (ed), Borrelia: molecular biology, host interaction and pathogenesis. Caister Academic Press, Norfolk, UK."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-011112-112140"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3016-4"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12609"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3553-5"},{"key":"e_1_3_2_13_2","first-page":"251","volume-title":"Borrelia: molecular biology, host interaction and pathogenesis","author":"Piesman J","year":"2010","unstructured":"Piesman J, Schwan TG. 2010. Ecology of borreliae and their arthropod vectors, p 251\u2013278. In Samuels DS, Radolf JD (ed), Borrelia: molecular biology, host interaction and pathogenesis. Caister Academic Press, Norfolk, UK."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1139\/o95-086"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00665-7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3331"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00742"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.11.3766-3774.1992"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80282-7"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-5-871"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.11.3757-3765.1992"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90833-8"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.9.2427-2434.1995"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1016\/S0021-9258(19)41734-1","article-title":"Escherichia coli ribosomal ribonucleic acids are not cut from an intact precursor molecule","volume":"250","author":"Gegenheimer P","year":"1975","unstructured":"Gegenheimer P, Apirion D. 1975. Escherichia coli ribosomal ribonucleic acids are not cut from an intact precursor molecule. J Biol Chem 250:2407\u20132409.","journal-title":"J Biol Chem"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.11.120177.001501"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(81)80984-2"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.000541"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)00809-X"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-4-13"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.2.445-452.2006"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.6165-6168.2005"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a003877"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2012.02632.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1201"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99327-0"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110711-155618"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1195"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"3361","DOI":"10.1073\/pnas.70.12.3361","article-title":"Synthesis of a large precursor to ribosomal RNA in a mutant of Escherichia coli","volume":"70","author":"Nikolaev N","year":"1973","unstructured":"Nikolaev N, Silengo L, Schlessinger D. 1973. Synthesis of a large precursor to ribosomal RNA in a mutant of Escherichia coli. Proc Natl Acad Sci U S A 70:3361\u20133365.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"7967","DOI":"10.1016\/S0021-9258(19)43281-X","article-title":"A role for ribonuclease III in processing of ribosomal ribonucleic acid and messenger ribonucleic acid precursors in Escherichia coli","volume":"248","author":"Nikolaev N","year":"1973","unstructured":"Nikolaev N, Silengo L, Schlessinger D. 1973. A role for ribonuclease III in processing of ribosomal ribonucleic acid and messenger ribonucleic acid precursors in Escherichia coli. J Biol Chem 248:7967\u20137969.","journal-title":"J Biol Chem"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02185.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/8.8.1873"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00242.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-18-23"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-120213-092340"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13295"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/S0021-9258(17)40663-6","article-title":"Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis","volume":"252","author":"Sogin ML","year":"1977","unstructured":"Sogin ML, Pace B, Pace NR. 1977. Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis. J Biol Chem 252:1350\u20131357.","journal-title":"J Biol Chem"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86643-7"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"3064","DOI":"10.1016\/S0021-9258(17)40472-8","article-title":"Multiple pathways for primary processing of ribosomal RNA in Escherichia coli","volume":"252","author":"Gegenheimer P","year":"1977","unstructured":"Gegenheimer P, Watson N, Apirion D. 1977. Multiple pathways for primary processing of ribosomal RNA in Escherichia coli. J Biol Chem 252:3064\u20133073.","journal-title":"J Biol Chem"},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"12034","DOI":"10.1016\/S0021-9258(17)44336-5","article-title":"S1 nuclease mapping analysis of ribosomal RNA processing in wild type and processing deficient Escherichia coli","volume":"258","author":"King TC","year":"1983","unstructured":"King TC, Schlessinger D. 1983. S1 nuclease mapping analysis of ribosomal RNA processing in wild type and processing deficient Escherichia coli. J Biol Chem 258:12034\u201312042.","journal-title":"J Biol Chem"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1073\/pnas.81.1.185","article-title":"RNase III cleavage is obligate for maturation but not for function of Escherichia coli pre-23S rRNA","volume":"81","author":"King TC","year":"1984","unstructured":"King TC, Sirdeshmukh R, Schlessinger D. 1984. RNase III cleavage is obligate for maturation but not for function of Escherichia coli pre-23S rRNA. Proc Natl Acad Sci U S A 81:185\u2013188.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.1.229-239.1993"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/228235a0"},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.1073\/pnas.70.12.3296","article-title":"T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease III","volume":"70","author":"Dunn JJ","year":"1973","unstructured":"Dunn JJ, Studier FW. 1973. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease III. Proc Natl Acad Sci U S A 70:3296\u20133300.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003181"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07374.x"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.4161\/21541272.2014.944039"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)42542-0"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(08)02207-6"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1999.tb00405.x"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00659-13"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600572"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.603108"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01467-12"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000680"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000679"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022168"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-011-0740-7"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00099-12"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002782"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-17"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07351.x"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.11.025"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2139-2150.2002"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231442498"},{"key":"e_1_3_2_75_2","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour AG","year":"1984","unstructured":"Barbour AG. 1984. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med 57:521\u2013525.","journal-title":"Yale J Biol Med"},{"key":"e_1_3_2_76_2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/978-1-4939-7383-5_15","volume-title":"Borrelia burgdorferi: methods and protocols","author":"Samuels DS","year":"2018","unstructured":"Samuels DS, Drecktrah D, Hall LS. 2018. Genetic transformation and complementation, p 183\u2013200. In Pal U, Buyuktanir O (ed), Borrelia burgdorferi: methods and protocols, vol 1690. Humana Press, New York, NY."},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.10.4354-4357.2005"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2012.00996.x"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005160"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068799"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031960"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.25.13865"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn757"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg120"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00035-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00035-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:45:05Z","timestamp":1627580705000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00035-18"}},"subtitle":[],"editor":[{"given":"Tina M.","family":"Henkin","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":84,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1128\/JB.00035-18"],"URL":"https:\/\/doi.org\/10.1128\/jb.00035-18","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"2018-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}