{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:26:42Z","timestamp":1790076402650,"version":"4.0.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI077628"],"award-info":[{"award-number":["R01AI077628"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI081983"],"award-info":[{"award-number":["R01AI081983"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM59026"],"award-info":[{"award-number":["R01GM59026"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Members of the genus\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia<\/jats:named-content>\n            are known to be adept at biofilm formation, which presumably assists in the survival of these organisms in the environment and the host. Biofilm formation has been linked to quorum sensing (QS) in several bacterial species. In this study, we characterized\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia thailandensis<\/jats:named-content>\n            biofilm development under flow conditions and sought to determine whether QS contributes to this process.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            biofilm formation exhibited an unusual pattern: the cells formed small aggregates and then proceeded to produce mature biofilms characterized by \u201cdome\u201d structures filled with biofilm matrix material. We showed that this process was dependent on QS.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            has three acyl-homoserine lactone (AHL) QS systems (QS-1, QS-2, and QS-3). An AHL-negative strain produced biofilms consisting of cell aggregates but lacking the matrix-filled dome structures. This phenotype was rescued via exogenous addition of the three AHL signals. Of the three\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            QS systems, we show that QS-1 is required for proper biofilm development, since a\n            <jats:italic>btaR1<\/jats:italic>\n            mutant, which is defective in QS-1 regulation, forms biofilms without these dome structures. Furthermore, our data show that the wild-type biofilm biomass, as well as the material inside the domes, stains with a fucose-binding lectin. The\n            <jats:italic>btaR1<\/jats:italic>\n            mutant biofilms, however, are negative for fucose staining. This suggests that the QS-1 system regulates the production of a fucose-containing exopolysaccharide in wild-type biofilms. Finally, we present data showing that QS ability during biofilm development produces a biofilm that is resistant to dispersion under stress conditions.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            The saprophyte\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia thailandensis<\/jats:named-content>\n            is a close relative of the pathogenic bacterium\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            , the causative agent of melioidosis, which is contracted from its environmental reservoir. Since most bacteria in the environment reside in biofilms,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            is an ideal model organism for investigating questions in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia<\/jats:named-content>\n            physiology. In this study, we characterized\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            biofilm development and sought to determine if quorum sensing (QS) contributes to this process. Our work shows that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            produces biofilms with unusual dome structures under flow conditions. Our findings suggest that these dome structures are filled with a QS-regulated, fucose-containing exopolysaccharide that may be involved in the resilience of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. thailandensis<\/jats:named-content>\n            biofilms against changes in the nutritional environment.\n          <\/jats:p>","DOI":"10.1128\/jb.00047-16","type":"journal-article","created":{"date-parts":[[2016,4,12]],"date-time":"2016-04-12T03:24:39Z","timestamp":1460431479000},"page":"2643-2650","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Quorum Sensing Influences Burkholderia thailandensis Biofilm Development and Matrix Production"],"prefix":"10.1128","volume":"198","author":[{"given":"Boo Shan","family":"Tseng","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Washington, School of Medicine, Seattle, Washington, USA"},{"name":"School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, Nevada, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte D.","family":"Majerczyk","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, School of Medicine, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Passos da Silva","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, School of Medicine, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine R.","family":"Chandler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, School of Medicine, Seattle, Washington, USA"},{"name":"Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Peter","family":"Greenberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, School of Medicine, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Parsek","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, School of Medicine, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro821"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4350-4360.2004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00591-09"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00200-09"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01405-13"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.50.1.727"},{"key":"e_1_3_3_8_2","first-page":"67","article-title":"Quorum sensing and microbial biofilms","volume":"322","author":"Irie Y","year":"2008","unstructured":"Irie Y, Parsek MR. 2008. 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