{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:59:17Z","timestamp":1761238757381,"version":"3.40.5"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T00:00:00Z","timestamp":1598313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K24 AI04831","R01 AI053674"],"award-info":[{"award-number":["K24 AI04831","R01 AI053674"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R44 AI068185"],"award-info":[{"award-number":["R44 AI068185"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI099269"],"award-info":[{"award-number":["R01 AI099269"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R41 AI122433"],"award-info":[{"award-number":["R41 AI122433"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2020,8,25]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            effector toxin translocation into host innate immune cells is critical for the establishment and dissemination of\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            infections. The medical need for new anti-\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            agents is evident by the fact that\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            ventilator-associated pneumonia is associated with a high mortality rate (40 to 69%) and recurs in &gt;30% of patients, even with standard-of-care antibiotic therapy. The results described here confirm roles for the PscF needle in T3SS secretion and translocation and suggest that it affects regulation, possibly by interaction with T3SS regulatory proteins. The results also support a model of direct interaction of the needle with PhA and suggest that, with further development, members of the PhA series may prove useful as drugs for\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/jb.00055-20","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T13:13:23Z","timestamp":1593090803000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["A Structure-Function-Inhibition Analysis of the\n            <i>Pseudomonas aeruginosa<\/i>\n            Type III Secretion Needle Protein PscF"],"prefix":"10.1128","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8625-0312","authenticated-orcid":false,"given":"Donald T.","family":"Moir","sequence":"first","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas O.","family":"Bowlin","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan J.","family":"Berube","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Northwestern University, Chicago, Illinois, USA"},{"name":"Department of Medicine, Northwestern University, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaden","family":"Yabut","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debra M.","family":"Mills","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giang T.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Tufts Graduate School in Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary D.","family":"Aron","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Williams","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9455-6672","authenticated-orcid":false,"given":"Joan","family":"Mecsas","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Hauser","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Northwestern University, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry L.","family":"Bowlin","sequence":"additional","affiliation":[{"name":"Microbiotix, Inc., Worcester, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2199"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.03.015"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.12.007"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00013-07"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.6969-6977.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00297"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201210-1897OC"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.109.4.1019"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/339488"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000275389.31974.22"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13063-017-1780-3"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.290.19.2588"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000249826.81273.E4"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000000510"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv436"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e318183f3f8"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/320737"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064867"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.07.006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00223-07"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1074-5521(03)00046-2"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00364-10"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01598-09"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.04.014"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00815-08"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/341069"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2015.01.011"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.09.028"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02795-13"},{"key":"e_1_3_3_31_2","article-title":"The structure of the type III secretion system needle complex","author":"Miletic S","year":"2019","unstructured":"Miletic S, Goessweiner-Mohr N, Marlovits TC. 31 October 2019. The structure of the type III secretion system needle complex. Curr Top Microbiol Immunol Epub ahead of print.","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0545-z"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3682"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0039-2018"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11079"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5976"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00573"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04790.x"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508377200"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602689103"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00004"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07096.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01396-06"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02011-12"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003245"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2004.10.005"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018356"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/7391"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00088-17"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508089200"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3104-3114.2005"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01680-06"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12069"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006985107"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.233858"},{"key":"e_1_3_3_56_2","volume-title":"Abstr ASM Microbe 2017","author":"Aron ZD","year":"2017","unstructured":"Aron ZD, Torhan MC, Bowlin NO, Williams JD, Bowlin TL, Moir DT. 2017. The potency of dimeric inhibitors of T3SS is consistent with a polymeric target. Abstr ASM Microbe 2017. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3232"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610098104"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503005102"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504405102"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.06.001"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1441"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1534-5807(03)00019-4"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1177\/1087057107304729"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.24"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1177\/1087057111408605"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01202-17"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/8.3.275"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00055-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00055-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:57:44Z","timestamp":1627581464000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00055-20"}},"subtitle":[],"editor":[{"given":"Thomas J.","family":"Silhavy","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,8,25]]},"references-count":67,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2020,8,25]]}},"alternative-id":["10.1128\/JB.00055-20"],"URL":"https:\/\/doi.org\/10.1128\/jb.00055-20","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"type":"print","value":"0021-9193"},{"type":"electronic","value":"1098-5530"}],"subject":[],"published":{"date-parts":[[2020,8,25]]},"assertion":[{"value":"2020-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}