{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:37:02Z","timestamp":1789627022918,"version":"build-2803163510"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T00:00:00Z","timestamp":1276560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The bacterial pathogen\n            <jats:italic>Brucella abortus<\/jats:italic>\n            was recently demonstrated to recruit the essential cytoplasmic histidine kinase PdhS to its old pole. Here, we report identification of the fumarase FumC as a specific partner for the N-terminal \u201csensing\u201d domain of PdhS, using an ORFeome-based yeast two-hybrid screen. We observed that FumC and PdhS colocalize at the old pole of\n            <jats:italic>B. abortus<\/jats:italic>\n            , while the other fumarase FumA is not polarly localized. FumC is not required for PdhS localization, and polar FumC localization is not FumA dependent. FumC homologs are not polarly localized in\n            <jats:italic>Sinorhizobium meliloti<\/jats:italic>\n            and\n            <jats:italic>Caulobacter crescentus<\/jats:italic>\n            , suggesting that polar recruitment of FumC by PdhS is evolutionarily recent.\n          <\/jats:p>","DOI":"10.1128\/jb.00066-10","type":"journal-article","created":{"date-parts":[[2010,4,10]],"date-time":"2010-04-10T01:09:58Z","timestamp":1270861798000},"page":"3235-3239","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["PdhS, an Old-Pole-Localized Histidine Kinase, Recruits the Fumarase FumC in\n            <i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Johann","family":"Mignolet","sequence":"first","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Van der Henst","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301cile","family":"Nicolas","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michae\u0308l","family":"Deghelt","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Dotreppe","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Molecular Biology Research Unit (URBM), University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.mib.2003.11.001","volume":"7","year":"2004","unstructured":"Celli, J., and J. P. Gorvel. 2004. Organelle robbery: Brucella interactions with the endoplasmic reticulum. Curr. Opin. Microbiol. 7 : 93-97.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00749.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1101\/gr.2456204","volume":"14","year":"2004","unstructured":"Dricot, A., J. F. Rual, P. Lamesch, N. Bertin, D. Dupuy, T. Hao, C. Lambert, R. Hallez, J. M. Delroisse, J. Vandenhaute, I. Lopez-Goni, I. Moriyon, J. M. Garcia-Lobo, F. J. Sangari, A. P. Macmillan, S. J. Cutler, A. M. Whatmore, S. Bozak, R. Sequerra, L. Doucette-Stamm, M. Vidal, D. E. Hill, J. J. Letesson, and X. De Bolle. 2004. Generation of the Brucella melitensis ORFeome version 1.1. Genome Res. 14 : 2201-2206.","journal-title":"Genome Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01834-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01923-05"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.tim.2004.06.002","volume":"12","year":"2004","unstructured":"Hallez, R., A. F. Bellefontaine, J. J. Letesson, and X. De Bolle. 2004. Morphological and functional asymmetry in alpha-proteobacteria. Trends Microbiol. 12 : 361-365.","journal-title":"Trends Microbiol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1038\/sj.emboj.7601577","volume":"26","year":"2007","unstructured":"Hallez, R., J. Mignolet, V. Van Mullem, M. Wery, J. Vandenhaute, J. J. Letesson, C. Jacobs-Wagner, and X. De Bolle. 2007. The asymmetric distribution of the essential histidine kinase PdhS indicates a differentiation event in Brucella abortus. EMBO J. 26 : 1444-1455.","journal-title":"EMBO J."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1042\/bj2370547","volume":"237","year":"1986","unstructured":"Woods, S. A., J. S. Miles, R. E. Roberts, and J. R. Guest. 1986. Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12. Biochem. J. 237 : 547-557.","journal-title":"Biochem. J."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/0167-4838(88)90050-7","volume":"954","year":"1988","unstructured":"Woods, S. A., S. D. Schwartzbach, and J. R. Guest. 1988. Two biochemically distinct classes of fumarase in Escherichia coli. Biochim. Biophys. Acta 954 : 14-26.","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00066-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00066-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:13:15Z","timestamp":1627578795000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00066-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,15]]},"references-count":9,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,6,15]]}},"alternative-id":["10.1128\/JB.00066-10"],"URL":"https:\/\/doi.org\/10.1128\/jb.00066-10","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,15]]},"assertion":[{"value":"2010-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}