{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:21:15Z","timestamp":1790904075875,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2006,7,15]],"date-time":"2006-07-15T00:00:00Z","timestamp":1152921600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Brucella<\/jats:italic>\n                    periplasmic cyclic \u03b2-1,2-glucan plays an important role during bacterium-host interaction. Nuclear magnetic resonance spectrometry analysis, thin-layer chromatography, and DEAE-Sephadex chromatography were used to characterize\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    cyclic glucan. In the present study, we report that a fraction of\n                    <jats:italic>B. abortus<\/jats:italic>\n                    cyclic \u03b2-1,2-glucan is substituted with succinyl residues, which confer anionic character on the cyclic \u03b2-1,2-glucan. The oligosaccharide backbone is substituted at C-6 positions with an average of two succinyl residues per glucan molecule. This O-ester-linked succinyl residue is the only substituent of\n                    <jats:italic>Brucella<\/jats:italic>\n                    cyclic glucan. A\n                    <jats:italic>B. abortus<\/jats:italic>\n                    open reading frame (BAB1_1718) homologous to\n                    <jats:italic>Rhodobacter sphaeroides<\/jats:italic>\n                    glucan succinyltransferase (OpgC) was identified as the gene encoding the enzyme responsible for cyclic glucan modification. This gene was named\n                    <jats:italic>cgm<\/jats:italic>\n                    for\n                    <jats:italic>c<\/jats:italic>\n                    yclic\n                    <jats:italic>g<\/jats:italic>\n                    lucan\n                    <jats:italic>m<\/jats:italic>\n                    odifier and is highly conserved in\n                    <jats:italic>Brucella melitensis<\/jats:italic>\n                    and\n                    <jats:italic>Brucella suis<\/jats:italic>\n                    . Nucleotide sequencing revealed that\n                    <jats:italic>B. abortus cgm<\/jats:italic>\n                    consists of a 1,182-bp open reading frame coding for a predicted membrane protein of 393 amino acid residues (42.7 kDa) 39% identical to\n                    <jats:italic>Rhodobacter sphaeroides<\/jats:italic>\n                    succinyltransferase.\n                    <jats:italic>cgm<\/jats:italic>\n                    null mutants in\n                    <jats:italic>B. abortus<\/jats:italic>\n                    strains 2308 and S19 produced neutral glucans without succinyl residues, confirming the identity of this protein as the cyclic-glucan succinyltransferase enzyme. In this study, we demonstrate that succinyl substituents of cyclic \u03b2-1,2-glucan of\n                    <jats:italic>B. abortus<\/jats:italic>\n                    are necessary for hypo-osmotic adaptation. On the other hand, intracellular multiplication and mouse spleen colonization are not affected in\n                    <jats:italic>cgm<\/jats:italic>\n                    mutants, indicating that cyclic-\u03b2-1,2-glucan succinylation is not required for virulence and suggesting that no low-osmotic stress conditions must be overcome during infection.\n                  <\/jats:p>","DOI":"10.1128\/jb.00086-06","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T19:17:58Z","timestamp":1151695078000},"page":"5003-5013","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["The\n                    <i>Brucella abortus<\/i>\n                    Cyclic \u03b2-1,2-Glucan Virulence Factor Is Substituted with O-Ester-Linked Succinyl Residues"],"prefix":"10.1128","volume":"188","author":[{"given":"Mara S.","family":"Roset","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas-Instituto Tecnolo\u0301gico de Chascomu\u0301s (IIB-INTECH), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Universidad Nacional de General San Marti\u0301n (CONICET-UNSAM), 1650 San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301s E.","family":"Ciocchini","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas-Instituto Tecnolo\u0301gico de Chascomu\u0301s (IIB-INTECH), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Universidad Nacional de General San Marti\u0301n (CONICET-UNSAM), 1650 San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas-Instituto Tecnolo\u0301gico de Chascomu\u0301s (IIB-INTECH), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Universidad Nacional de General San Marti\u0301n (CONICET-UNSAM), 1650 San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"In\u0303o\u0301n de Iannino","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas-Instituto Tecnolo\u0301gico de Chascomu\u0301s (IIB-INTECH), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Universidad Nacional de General San Marti\u0301n (CONICET-UNSAM), 1650 San Marti\u0301n, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"390","year":"1998","unstructured":"Altabe, S. 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