{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:08:37Z","timestamp":1785251317042,"version":"3.55.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Knut and Alice Wallenburg Foundation"},{"name":"Kempe and the Swedish Research Council"},{"name":"Laboratory for Molecular Infection Medicine Sweden"},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["2P20GM103432"],"award-info":[{"award-number":["2P20GM103432"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB-1518171"],"award-info":[{"award-number":["MCB-1518171"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS1557806"],"award-info":[{"award-number":["IOS1557806"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Agrobacterium tumefaciens<\/jats:named-content>is a rod-shaped bacterium that grows by polar insertion of new peptidoglycan during cell elongation. As the cell cycle progresses, peptidoglycan synthesis at the pole ceases prior to insertion of new peptidoglycan at midcell to enable cell division. The<jats:named-content content-type=\"genus-species\">A. tumefaciens<\/jats:named-content>homolog of the<jats:named-content content-type=\"genus-species\">Caulobacter crescentus<\/jats:named-content>polar organelle development protein PopZ has been identified as a growth pole marker and a candidate polar growth-promoting factor. Here, we characterize the function of PopZ in cell growth and division of<jats:named-content content-type=\"genus-species\">A. tumefaciens<\/jats:named-content>. Consistent with previous observations, we observe that PopZ localizes specifically to the growth pole in wild-type cells. Despite the striking localization pattern of PopZ, we find the absence of the protein does not impair polar elongation or cause major changes in the peptidoglycan composition. Instead, we observe an atypical cell length distribution, including minicells, elongated cells, and cells with ectopic poles. Most minicells lack DNA, suggesting a defect in chromosome segregation. Furthermore, the canonical cell division proteins FtsZ and FtsA are misplaced, leading to asymmetric sites of cell constriction. Together, these data suggest that PopZ plays an important role in the regulation of chromosome segregation and cell division.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold><jats:named-content content-type=\"genus-species\">A. tumefaciens<\/jats:named-content>is a bacterial plant pathogen and a natural genetic engineer. However, very little is known about the spatial and temporal regulation of cell wall biogenesis that leads to polar growth in this bacterium. Understanding the molecular basis of<jats:named-content content-type=\"genus-species\">A. tumefaciens<\/jats:named-content>growth may allow for the development of innovations to prevent disease or to promote growth during biotechnology applications. Finally, since many closely related plant and animal pathogens exhibit polar growth, discoveries in<jats:named-content content-type=\"genus-species\">A. tumefaciens<\/jats:named-content>may be broadly applicable for devising antimicrobial strategies.<\/jats:p>","DOI":"10.1128\/jb.00101-17","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:30:35Z","timestamp":1497918635000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Absence of the Polar Organizing Protein PopZ Results in Reduced and Asymmetric Cell Division in Agrobacterium tumefaciens"],"prefix":"10.1128","volume":"199","author":[{"given":"Matthew","family":"Howell","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Aliashkevich","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5 Center for Microbial Research, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne K.","family":"Salisbury","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Wyoming, Laramie, Wyoming, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Cava","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5 Center for Microbial Research, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant R.","family":"Bowman","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Wyoming, Laramie, Wyoming, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J. 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