{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:27:42Z","timestamp":1785414462022,"version":"3.56.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/13410-1"],"award-info":[{"award-number":["2022\/13410-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/25682-0"],"award-info":[{"award-number":["2018\/25682-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014456","name":"Center for Bioenergy Innovation","doi-asserted-by":"publisher","award":["ERKP886"],"award-info":[{"award-number":["ERKP886"]}],"id":[{"id":"10.13039\/100014456","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,7,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Thermoanaerobacterium saccharolyticum<\/jats:italic>\n                      is a promising candidate for the production of biofuels from lignocellulosic sugars; however, the genes associated with electron transfer from ferredoxin are poorly characterized. In this work, we deleted several key electron transfer genes. We showed that the\n                      <jats:italic toggle=\"yes\">tsac_1705<\/jats:italic>\n                      gene is not necessary for high-yield ethanol production, but that a set of four other genes (\n                      <jats:italic toggle=\"yes\">nfnA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">nfnB<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">hfsD<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">hydA<\/jats:italic>\n                      ) are necessary. We showed that the\n                      <jats:italic toggle=\"yes\">nfnB<\/jats:italic>\n                      gene can function as a monofunctional (i.e., non-bifurcating) FNOR enzyme in the absence of\n                      <jats:italic toggle=\"yes\">nfnA<\/jats:italic>\n                      . The phenotypes of the\n                      <jats:italic toggle=\"yes\">hfsD<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">hydA<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">hfsD hydA<\/jats:italic>\n                      double-deletion strains are consistent with their function via hydrogen cycling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The improved understanding of electron transfer pathways in\n                      <jats:italic toggle=\"yes\">T. saccharolyticum<\/jats:italic>\n                      will enable future efforts to transfer the robust ethanol production pathway from this microbe to other organisms, with potential implications for industrial biofuel production.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00107-25","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T09:02:02Z","timestamp":1749200522000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Identification of electron transfer enzymes in\n                    <i>Thermoanaerobacterium saccharolyticum<\/i>"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-1861","authenticated-orcid":false,"given":"Jo\u00e3o H. 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