{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:27:10Z","timestamp":1792787230459,"version":"4.5.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T00:00:00Z","timestamp":1589155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T00:00:00Z","timestamp":1589155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"K.A. Wallenberg foundation"},{"DOI":"10.13039\/501100004885","name":"Ume\u00e5 University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004885","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Svenska Forskningsr\u00e5det","award":["2016-03313"],"award-info":[{"award-number":["2016-03313"]}]},{"DOI":"10.13039\/501100004200","name":"Stiftelsen Olle Engkvist Byggm\u00e4stare","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004200","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2020,5,11]]},"abstract":"<jats:p>\n                    The Gram-positive bacterium\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    is a human pathogen affecting mainly the elderly, immunocompromised people, and pregnant women. It can lead to meningoencephalitis, septicemia, and abortion. The major virulence regulator in\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    is the PrfA protein, a transcriptional activator. Using a growth-based selection strategy, we identified mutations in the PrfA protein leading to constitutively active virulence factor expression. We provide structural evidence for the existence of an intermediately activated PrfA state, which gives new insights into PrfA protein activation.\n                  <\/jats:p>","DOI":"10.1128\/jb.00115-20","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T08:30:06Z","timestamp":1584088206000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes"],"prefix":"10.1128","volume":"202","author":[{"given":"Sabine","family":"Hansen","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Ume\u00e5 Center for Microbial Research (UCMR), Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Ume\u00e5 Center for Microbial Research (UCMR), Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin","family":"Grundstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Ume\u00e5 Center for Microbial Research (UCMR), Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristoffer","family":"Br\u00e4nnstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0124-0199","authenticated-orcid":false,"given":"A. 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