{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:24:34Z","timestamp":1791591874520,"version":"4.3.4"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2019,6,15]],"date-time":"2019-06-15T00:00:00Z","timestamp":1560556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI107159"],"award-info":[{"award-number":["R01AI107159"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI107792"],"award-info":[{"award-number":["U19AI107792"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2019,6,15]]},"abstract":"<jats:p>\n                    <jats:italic>Brucella<\/jats:italic>\n                    species cause brucellosis, a global zoonosis. A gene encoding a conserved DUF1849-family protein, which we have named EipB, is present in all sequenced\n                    <jats:italic>Brucella<\/jats:italic>\n                    and several other genera in the class\n                    <jats:italic>Alphaproteobacteria<\/jats:italic>\n                    . The manuscript provides the first functional and structural characterization of a DUF1849 protein. We show that EipB is secreted to the periplasm where it forms a spiral-shaped antiparallel \u03b2 protein that is a determinant of cell envelope integrity\n                    <jats:italic>in vitro<\/jats:italic>\n                    and virulence in an animal model of disease.\n                    <jats:italic>eipB<\/jats:italic>\n                    genetically interacts with\n                    <jats:italic>ttpA<\/jats:italic>\n                    , which also encodes a periplasmic protein. We propose that EipB and TtpA function as part of a system required for cell envelope homeostasis in select\n                    <jats:italic>Alphaproteobacteria<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/jb.00134-19","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T09:05:13Z","timestamp":1553850313000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["<i>Brucella<\/i>\n                    Periplasmic Protein EipB Is a Molecular Determinant of Cell Envelope Integrity and Virulence"],"prefix":"10.1128","volume":"201","author":[{"given":"Julien","family":"Herrou","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan W.","family":"Willett","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0612-5029","authenticated-orcid":false,"given":"Aretha","family":"Fiebig","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel M.","family":"Czy\u017c","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason X.","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Pathology, The University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eveline","family":"Ultee","sequence":"additional","affiliation":[{"name":"Department of Biology, Universiteit Leiden, Leiden, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Briegel","sequence":"additional","affiliation":[{"name":"Department of Biology, Universiteit Leiden, Leiden, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance","family":"Bigelow","sequence":"additional","affiliation":[{"name":"Biosciences Division, Argonne National Laboratory, Argonne, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy","family":"Babnigg","sequence":"additional","affiliation":[{"name":"Biosciences Division, Argonne National Laboratory, Argonne, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngchang","family":"Kim","sequence":"additional","affiliation":[{"name":"Biosciences Division, Argonne National Laboratory, Argonne, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1727-322X","authenticated-orcid":false,"given":"Sean","family":"Crosson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00214-6"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1044"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102905"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-009-0123-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-021815-111326"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060636a"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14178"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1344"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0124-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.7053-7060.2003"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.082420-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.4049\/jimmunol.144.5.1629","article-title":"Heterogeneity of mouse helper T cells: evidence from bulk cultures and limiting dilution cloning for precursors of Th1 and Th2 cells","volume":"144","author":"Street NE","year":"1990","unstructured":"Street NE, Schumacher JH, Fong TA, Bass H, Fiorentino DF, Leverah JA, Mosmann TR. 1990. 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