{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:44:26Z","timestamp":1788187466801,"version":"build-2803163510"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","award":["CRC 2021-00103"],"award-info":[{"award-number":["CRC 2021-00103"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,8,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The PA7-clade (or group 3) of\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      is now recognized as a distinct species,\n                      <jats:italic toggle=\"yes\">Pseudomonas paraeruginosa<\/jats:italic>\n                      . We report here the genomic sequences of six new strains of\n                      <jats:italic toggle=\"yes\">P. paraeruginosa<\/jats:italic>\n                      : Zw26 (the first complete genome of a cystic fibrosis isolate of\n                      <jats:italic toggle=\"yes\">P. paraeruginosa<\/jats:italic>\n                      )\n                      <jats:italic toggle=\"yes\">,<\/jats:italic>\n                      draft genomes of four burn and wound strains from Argentina very closely related to PA7, and of Pa5196, the strain in which arabinosylation of type IV pili was documented. We compared the genomes of 82 strains of\n                      <jats:italic toggle=\"yes\">P. paraeruginosa<\/jats:italic>\n                      and confirmed that the species is divided into two sub-clades. Core genomes are very similar, while most differences are found in \u201cregions of genomic plasticity\u201d (RGPs). Several genomic deletions were identified, and most are common to the CR1 sub-clade that includes Zw26 and Pa5196. All strains lack the type 3 secretion system (T3SS) and instead use an alternative virulence strategy involving an exolysin, a characteristic shared with group 5\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">aeruginosa<\/jats:italic>\n                      . All strains tend to be multiresistant like PA7, with a significant proportion of carbapenem-resistant strains, either\n                      <jats:italic toggle=\"yes\">oprD<\/jats:italic>\n                      mutants or carrying carbapenemase genes. Although\n                      <jats:italic toggle=\"yes\">P. paraeruginosa<\/jats:italic>\n                      is still relatively rare, it has a worldwide distribution. Its multiresistance and its alternative virulence strategy need to be considered in future therapeutic development.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        is an important opportunistic pathogen causing respiratory infections, notably in cystic fibrosis, and burn and wound infections. Our study reports six new genomes of\n                        <jats:italic toggle=\"yes\">Pseudomonas paraeruginosa<\/jats:italic>\n                        , a new species recently reported as distinct from\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        . The number of sequenced genomes of\n                        <jats:italic toggle=\"yes\">P. paraeruginosa<\/jats:italic>\n                        is only about 1% that of\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        . We compare the genomic content of nearly all strains of\n                        <jats:italic toggle=\"yes\">P. paraeruginosa<\/jats:italic>\n                        in GenBank, highlighting the differences in core and accessory genomes, antimicrobial resistance genes, and virulence factors. This novel species is very similar in environmental spectrum to\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        but is notably resistant to last-line antibiotics and uses an alternative virulence strategy based on exolysin\u2014this strategy being shared with some\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        outliers.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00149-25","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T13:00:26Z","timestamp":1753448426000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Comparative genomics of\n                    <i>Pseudomonas paraeruginosa<\/i>"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9094-0500","authenticated-orcid":false,"given":"Maxime","family":"D\u00e9raspe","sequence":"first","affiliation":[{"name":"Infectious and Immune Diseases Research Program, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center","place":["Qu\u00e9bec City, Canada"]},{"name":"Department of Molecular Medicine, Faculty of Medicine, Universit\u00e9 Laval","place":["Qu\u00e9bec City, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0838-5040","authenticated-orcid":true,"given":"Lori L.","family":"Burrows","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02fa3aq29","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Biomedical Sciences, and the Michael G. 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Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9973-2740","authenticated-orcid":false,"given":"Jacques","family":"Corbeil","sequence":"additional","affiliation":[{"name":"Infectious and Immune Diseases Research Program, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center","place":["Qu\u00e9bec City, Canada"]},{"name":"Department of Molecular Medicine, Faculty of Medicine, Universit\u00e9 Laval","place":["Qu\u00e9bec City, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3526-3997","authenticated-orcid":true,"given":"Paul H.","family":"Roy","sequence":"additional","affiliation":[{"name":"Infectious and Immune Diseases Research Program, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center","place":["Qu\u00e9bec City, Canada"]},{"id":[{"id":"https:\/\/ror.org\/04sjchr03","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Microbiology and 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