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FlhDC, the master regulator of flagellar genes, controls these traits by positively regulating<jats:italic>gacA<\/jats:italic>,<jats:italic>fliA<\/jats:italic>, and<jats:italic>rsmC<\/jats:italic>and negatively regulating<jats:italic>hexA<\/jats:italic>. RsmC, on the other hand, is a negative regulator of extracellular proteins\/enzymes, motility, and virulence since the deficiency of RsmC in FlhDC<jats:sup>+<\/jats:sup>strain results in overproduction of extracellular proteins\/enzymes, hypermotility, and hypervirulence. These phenotypes are abolished in an RsmC<jats:sup>\u2212<\/jats:sup>FlhDC<jats:sup>\u2212<\/jats:sup>double mutant. We show that RsmC interferes with FlhDC action. Indeed, the expression of all three targets (i.e.,<jats:italic>gacA<\/jats:italic>,<jats:italic>rsmC<\/jats:italic>, and<jats:italic>fliA<\/jats:italic>) positively regulated in<jats:italic>E<\/jats:italic>.<jats:italic>carotovora<\/jats:italic>subsp.<jats:italic>carotovora<\/jats:italic>by FlhDC is inhibited by RsmC. RsmC also partly relieves the inhibition of<jats:italic>hexA<\/jats:italic>expression by FlhDC. The results of yeast two-hybrid analysis revealed that RsmC binds FlhD and FlhDC, but not FlhC. We propose that binding of RsmC with FlhD\/FlhDC interferes with its regulatory functions and that RsmC acts as an anti-FlhD<jats:sub>4<\/jats:sub>FlhC<jats:sub>2<\/jats:sub>factor. We document here for the first time that RsmC interferes with activation of<jats:italic>fliA<\/jats:italic>and motility in several members of the<jats:italic>Enterobacteriaceae<\/jats:italic>family. The extent of<jats:italic>E<\/jats:italic>.<jats:italic>carotovora<\/jats:italic>subsp.<jats:italic>carotovora<\/jats:italic>RsmC-mediated inhibition of FlhDC-dependent expression of<jats:italic>fliA<\/jats:italic>and motility varies depending upon enterobacterial species. The data presented here support the idea that differences in structural features in enterobacterial FlhD are responsible for differential susceptibility to<jats:italic>E<\/jats:italic>.<jats:italic>carotovora<\/jats:italic>subsp.<jats:italic>carotovora<\/jats:italic>RsmC action.<\/jats:p>","DOI":"10.1128\/jb.00154-09","type":"journal-article","created":{"date-parts":[[2009,5,16]],"date-time":"2009-05-16T01:09:37Z","timestamp":1242436177000},"page":"4582-4593","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["RsmC of<i>Erwinia carotovora<\/i>subsp.<i>carotovora<\/i>Negatively Controls Motility, Extracellular Protein Production, and Virulence by Binding FlhD and Modulating Transcriptional Activity of the Master Regulator, FlhDC"],"prefix":"10.1128","volume":"191","author":[{"given":"Asita","family":"Chatterjee","sequence":"first","affiliation":[{"name":"Division of Plant Sciences, 108 Waters Hall, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaya","family":"Cui","sequence":"additional","affiliation":[{"name":"Division of Plant Sciences, 108 Waters Hall, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun K.","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Division of Plant Sciences, 108 Waters Hall, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1046\/j.1365-2443.1998.00219.x","volume":"3","year":"1998","unstructured":"Aizawa, S. 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