{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:31:44Z","timestamp":1785407504282,"version":"3.56.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Key R&D Program Topic: Research on New Quarantine and Detection Technologies for Important Emerging and Exotic Animal Diseases at Ports","award":["2021YFD1800503"],"award-info":[{"award-number":["2021YFD1800503"]}]},{"name":"Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences","award":["NULL"],"award-info":[{"award-number":["NULL"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,9,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycoplasma bovis<\/jats:italic>\n                      is a highly infectious pathogenic microorganism that causes various clinical signs in cattle, including pneumonia, arthritis, and mastitis, often resulting in significant economic losses. The adhesion of\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      to host cells is a pivotal step in the infection process, which is a complex process involving multiple pathogenic and host proteins. Molecules involved in\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      adhesion and colonization are widely recognized as important virulence factors and often implicated in the infection and pathogenesis. In this study, the\n                      <jats:italic toggle=\"yes\">Mbov_0510<\/jats:italic>\n                      gene of\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      was cloned, and the protein encoded by this gene was expressed and purified. The rabbit polyclonal antibody against this protein was also produced. This protein was shown to be a surface protein and to react with the\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      -positive serum. The ability of this protein to adhere to host cells was verified using embryonic bovine lung (EBL) and Madin-Darby bovine kidney (MDBK) cells. Furthermore, the adhesion function of this protein was found to be achieved through interaction with heparin on the host cell surface, and the key region of the protein involved in heparin binding was identified. The conservation of the protein encoded by the\n                      <jats:italic toggle=\"yes\">Mbov_0510<\/jats:italic>\n                      gene was also analyzed. The results of this study suggest that the protein encoded by the\n                      <jats:italic toggle=\"yes\">Mbov_0510<\/jats:italic>\n                      gene is a heparin-binding surface membrane protein of\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      associated with infection.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Mycoplasmas lack a cell wall, and the membrane proteins interacting with host cells play essential roles in their infection and proliferation processes. In this study, we identified a membrane protein encoded by\n                        <jats:italic toggle=\"yes\">Mycoplasma bovis<\/jats:italic>\n                        that interacts with heparin on the surface of host cells. Heparin is widely distributed in various cells and tissues of the host and serves as a receptor for the infection and invasion of many pathogenic microorganisms. The ability of\n                        <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                        to invade multiple tissues may be related to its heparin-binding capacity. The heparin-binding protein identified in this study is valuable for further research on the infection and invasion mechanisms of\n                        <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00160-25","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T13:21:05Z","timestamp":1754918465000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Identification of a heparin-binding protein encoded by\n                    <i>Mbov_0510<\/i>\n                    gene in\n                    <i>Mycoplasma bovis<\/i>"],"prefix":"10.1128","volume":"207","author":[{"given":"Zhixin","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5770-1595","authenticated-orcid":true,"given":"Tong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuqing","family":"Xin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0393-8726","authenticated-orcid":true,"given":"Qingyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Harbin, China"]},{"id":[{"id":"https:\/\/ror.org\/0313jb750","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Western Agriculture, Chinese Academy of Agricultural Sciences","place":["Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2007.11.012"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870601800106"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9080640"},{"key":"e_1_3_4_5_2","first-page":"863","article-title":"Coinfection with bovine viral diarrhea virus and Mycoplasma bovis in feedlot cattle with chronic pneumonia","volume":"43","author":"Shahriar FM","year":"2002","unstructured":"Shahriar FM, Clark EG, Janzen E, West K, Wobeser G. 2002. 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