{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:05Z","timestamp":1787016665977,"version":"build-2736575974"},"reference-count":84,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["DE014711-10"],"award-info":[{"award-number":["DE014711-10"]}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["P20GM103449"],"award-info":[{"award-number":["P20GM103449"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2023,9,26]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dental caries is among the most prevalent chronic diseases worldwide.\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    , the chief causative agent of caries, uses a 25-kDa manganese-dependent SloR protein to coordinate the uptake of essential manganese with the transcription of its virulence attributes. Small non-coding RNAs (sRNAs) can either enhance or repress gene expression, and reports in the literature ascribe an emerging role for sRNAs in the environmental stress response. Herein, we focused our attention on 18\u201350 nt sRNAs as mediators of the\n                    <jats:italic>S. mutans<\/jats:italic>\n                    SloR and manganese regulons. Specifically, the results of RNA sequencing revealed 19 sRNAs in\n                    <jats:italic>S. mutans,<\/jats:italic>\n                    which were differentially transcribed in the SloR-proficient UA159 and SloR-deficient GMS584 strains, and 10 sRNAs that were differentially expressed in UA159 cells grown in the presence of low vs high manganese. We describe SmsR1532 and SmsR1785 as SloR- and manganese-responsive sRNAs that are processed from large transcripts and that bind SloR directly in their promoter regions. The predicted targets of these sRNAs include regulators of metal ion transport, growth management via a toxin-antitoxin operon, and oxidative stress tolerance. These findings support a role for sRNAs in coordinating intracellular metal ion homeostasis with virulence gene control in an important oral cariogen.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Small regulatory RNAs (sRNAs) are critical mediators of environmental signaling, particularly in bacterial cells under stress, but their role in\n                      <jats:italic>Streptococcus mutans<\/jats:italic>\n                      is poorly understood.\n                      <jats:italic>S. mutans,<\/jats:italic>\n                      the principal causative agent of dental caries, uses a 25-kDa manganese-dependent protein, called SloR, to coordinate the regulated uptake of essential metal ions with the transcription of its virulence genes. In the present study, we identified and characterized sRNAs that are both SloR and manganese responsive. Taken together, this research can elucidate the details of regulatory networks that engage sRNAs in an important oral pathogen and that can enable the development of an effective anti-caries therapeutic.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00172-23","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T15:06:49Z","timestamp":1694444809000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Small regulatory RNAs are mediators of the\n                    <i>Streptococcus mutans<\/i>\n                    SloR regulon"],"prefix":"10.1128","volume":"205","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3566-634X","authenticated-orcid":true,"given":"India Y.","family":"Drummond","sequence":"first","affiliation":[{"name":"Department of Biology, Middlebury College , Middlebury, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"DePaolo","sequence":"additional","affiliation":[{"name":"Department of Biology, Middlebury College , Middlebury, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline","family":"Krieger","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University , Portland, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Driscoll","sequence":"additional","affiliation":[{"name":"Department of Biology, Vermont Biomedical Research Network, Norwich University , Northfield, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korin","family":"Eckstrom","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, The Robert Larner, M.D. College of Medicine, University of Vermont , Burlington, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2043-4853","authenticated-orcid":true,"given":"Grace A.","family":"Spatafora","sequence":"additional","affiliation":[{"name":"Department of Biology, Middlebury College , Middlebury, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374940080022001"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000016487"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26082-0"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172501299"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0051-2018"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.50.4.353-380.1986"},{"key":"e_1_3_4_8_2","first-page":"95","article-title":"Responses of cariogenic streptococci to environmental stresses","volume":"7","author":"Lemos JAC","year":"2005","unstructured":"Lemos JAC , Abranches J , Burne RA . 2005. 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