{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T23:21:01Z","timestamp":1776295261567,"version":"3.50.1"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI155424"],"award-info":[{"award-number":["R01 AI155424"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["JEAN21F0"],"award-info":[{"award-number":["JEAN21F0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2024,7,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The cystic fibrosis (CF) lung environment is conducive to the colonization of bacteria as polymicrobial biofilms, which are associated with poor clinical outcomes for persons with CF (pwCF).\n              <jats:italic>Streptococcus<\/jats:italic>\n              spp. are highly prevalent in the CF airway, but its role in the CF lung microbiome is poorly understood. Some studies have shown\n              <jats:italic>Streptococcus<\/jats:italic>\n              spp. to be associated with better clinical outcomes for pwCF, while others show that high abundance of\n              <jats:italic>Streptococcus<\/jats:italic>\n              spp. is correlated with exacerbations. Our lab previously reported a polymicrobial culture system consisting of four CF-relevant pathogens that can be used to study microbial behavior in a more clinically relevant setting. Here, we use this model system to identify genetic pathways that are important for\n              <jats:italic>Streptococcus sanguinis<\/jats:italic>\n              survival in the context of the polymicrobial community. We identified genes related to reactive oxygen species as differentially expressed in\n              <jats:italic>S. sanguinis<\/jats:italic>\n              monoculture versus growth of this microbe in the mixed community. Genetic studies identified Dpr as important for\n              <jats:italic>S. sanguinis<\/jats:italic>\n              survival in the community. We show that Dpr, a DNA-binding ferritin-like protein, and PerR, a peroxide-responsive transcriptional regulator of Dpr, are important for protecting\n              <jats:italic>S. sanguinis<\/jats:italic>\n              from phenazine-mediated toxicity in co-culture with\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n              and when exposed to hydrogen peroxide, both of which mimic the CF lung environment. Characterizing such interactions in a clinically relevant model system contributes to our understanding of microbial behavior in the context of polymicrobial biofilm infections.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Streptococcus<\/jats:italic>\n              spp. are recognized as a highly prevalent pathogen in cystic fibrosis (CF) airway infections. However, the role of this microbe in clinical outcomes for persons with CF is poorly understood. Here, we leverage a polymicrobial community system previously developed by our group to model CF airway infections as a tool to investigate a\n              <jats:italic>Pseudomonas<\/jats:italic>\n              -\n              <jats:italic>Streptococcus<\/jats:italic>\n              interaction involving reactive oxygen species (ROS). We show that protection against ROS is required for\n              <jats:italic>Streptococcus sanguinis<\/jats:italic>\n              survival in a clinically relevant polymicrobial system. Using this model system to study interspecies interactions contributes to our broader understanding of the complex role of\n              <jats:italic>Streptococcus<\/jats:italic>\n              spp. in the CF lung.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jb.00176-24","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T13:01:12Z","timestamp":1719579672000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Dpr-mediated H\n            <sub>2<\/sub>\n            O\n            <sub>2<\/sub>\n            resistance contributes to streptococcus survival in a cystic fibrosis airway model system"],"prefix":"10.1128","volume":"206","author":[{"given":"Rendi R.","family":"Rogers","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4990-4695","authenticated-orcid":true,"given":"Christopher A.","family":"Kesthely","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3056-8356","authenticated-orcid":true,"given":"Fabrice","family":"Jean-Pierre","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3277-2948","authenticated-orcid":true,"given":"Bassam","family":"El Hafi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, 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