{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T19:59:05Z","timestamp":1778011145454,"version":"3.51.4"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100006087","name":"UNAM | Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN203119"],"award-info":[{"award-number":["IN203119"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>\n            Protein concentration gradients play a relevant role in the organization of the bacterial cell. The\n            <jats:named-content content-type=\"genus-species\">Caulobacter crescentus<\/jats:named-content>\n            protein OmpA2 forms an outer membrane polar concentration gradient. To understand the molecular mechanism that determines the formation of this gradient, we characterized the mobility and localization of the full protein and of its two structural domains an integral outer membrane \u03b2-barrel and a periplasmic peptidoglycan binding domain. Each domain has a different role in the formation of the OmpA2 gradient, which occurs in two steps. We also show that the OmpA2 outer membrane \u03b2-barrel can diffuse, which is in contrast to what has been reported previously for several integral outer membrane proteins in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            , suggesting a different organization of the outer membrane proteins.\n          <\/jats:p>","DOI":"10.1128\/jb.00177-19","type":"journal-article","created":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T12:41:48Z","timestamp":1560516108000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Establishment of a Protein Concentration Gradient in the Outer Membrane Requires Two Diffusion-Limiting Mechanisms"],"prefix":"10.1128","volume":"201","author":[{"given":"Luis David","family":"Ginez","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurora","family":"Osorio","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0344-6000","authenticated-orcid":false,"given":"Laura","family":"Camarena","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Poggio","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000414"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.107.158101"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1970.tb00301.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1970.tb00936.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.184.3.754-759.2002"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.18.4872-4878.1998"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00778.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.136.1.280-285.1978"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1978.tb12771.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb05870.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/2983"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-188425"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.28.3.231-295.1964"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402606101"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20519"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2013.11.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01516-14"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2015.10.007"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160862"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-66"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14461"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.04.093"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00326-06"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04242.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.6.1913-1922.2005"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00220"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0035-2018"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002680"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.135.2.687-702.1978"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.143.2.906-913.1980"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90223-5"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0005-2736(98)00145-x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0005-2736(01)00410-2"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a131414"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.134.3.1181-1183.1978"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.1.197-203.1999"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.07.016"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.37.790"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05522.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.04.015"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2015.0023"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09152"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157899"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00572"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00521-17"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4024"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.710715"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1177\/1753425907087588"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.143.3.1215-1222.1980"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05720.x"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6728-6737.2004"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508536112"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01620-16"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01356.x"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00093-08"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049625"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090230"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000165"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2016.08.010"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)04019-K"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.2.488"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00811-16"},{"key":"e_1_3_3_64_2","doi-asserted-by":"crossref","DOI":"10.14440\/jbm.2014.36","article-title":"Advanced methods of microscope control using \u03bcManager software","volume":"1","author":"Edelstein AD","year":"2014","unstructured":"Edelstein AD, Tsuchida MA, Amodaj N, Pinkard H, Vale RD, Stuurman N. 2014. Advanced methods of microscope control using \u03bcManager software. J Biol Methods 1:e10.","journal-title":"J Biol Methods"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(83)84410-5"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00177-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00177-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:48:41Z","timestamp":1627580921000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00177-19"}},"subtitle":[],"editor":[{"given":"Yves V.","family":"Brun","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":65,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1128\/JB.00177-19"],"URL":"https:\/\/doi.org\/10.1128\/jb.00177-19","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"2019-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}