{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:35:54Z","timestamp":1792798554112,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100011773","name":"Region Bourgogne Franche-Comte","doi-asserted-by":"crossref","award":["2018Y-04631"],"award-info":[{"award-number":["2018Y-04631"]}],"id":[{"id":"10.13039\/501100011773","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Centre de Recherche Pseudomonas","award":["2024"],"award-info":[{"award-number":["2024"]}]},{"name":"French cystic fibrosis associations Vaincre la Mucoviscidose and Gregory Lemarchal","award":["RF20200502712"],"award-info":[{"award-number":["RF20200502712"]}],"id":[{"id":"https:\/\/ror.org\/00gp9bw49","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2025,10,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      In France, the use of essential oils to treat bacterial infections is common, with approximately 40% of cystic fibrosis patients regularly using these natural products to control infections caused by\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      often in combination with their antibiotic treatments. Previous research has demonstrated that prolonged exposure of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      PA14 to cinnamaldehyde (CNA), the main component of cinnamon essential oil, can select for mutants resistant to \u03b2-lactams through overproduction of the MexAB-OprM efflux pump, some of which are also hypersusceptible to aminoglycosides and colistin. We showed here that this hypersusceptibility is not due to an efflux defect, as the deletion of MexXY(OprM)\u2014the specific efflux pump for aminoglycosides\u2014still results in decreased minimum inhibitory concentrations of aminoglycosides. Genome sequencing of hypersusceptible mutants revealed mutations in the ATP synthase operon or its promoter (\n                      <jats:italic toggle=\"yes\">atpIBEFHAGDC<\/jats:italic>\n                      ). Surprisingly, although mutations in the\n                      <jats:italic toggle=\"yes\">atp<\/jats:italic>\n                      operon reduced bacterial growth and ATP production, they are not uncommon in clinical strains. We found that ATP synthase alterations modified the respiratory chain and led to inner membrane hyperpolarization, likely enhancing positively charged antibiotic (aminoglycosides and colistin) uptake and susceptibility to these molecules. In addition, the modified respiratory chain increased the proton motive force, allowing the overproduction of the MexAB-OpM efflux pump, which protects bacteria from CNA and from the clinically relevant \u03b2-lactam antibiotics. Altogether, these results indicate a trade-off between CNA resistance and aminoglycoside\/colistin susceptibility, a reaction that may question the survival of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      in the lung of CF patients possibly submitted to these therapeutic molecules.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>\n                        <jats:bold>IMPORTANCE<\/jats:bold>\n                      <\/jats:title>\n                      <jats:p>\n                        In France, essential oils are widely used by cystic fibrosis patients (40%), often alongside antibiotic therapies, to help control\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        infections. Cinnamaldehyde from cinnamon essential oil appears to select for\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        mutants that are resistant to \u03b2-lactam antibiotics due to the overproduction of the MexAB-OprM efflux pump and hypersusceptible to aminoglycosides and colistin. This increased susceptibility is associated with mutations in ATP synthase, which elevate the proton motive force (PMF) and facilitate both (i) increased uptake of positively charged antibiotics (aminoglycosides, colistin) and (ii) more efficient efflux of \u03b2-lactams via MexAB-OpM. Thus, the use of cinnamaldehyde may drive a trade-off in\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        between \u03b2-lactam resistance and aminoglycosides\/polymyxins susceptibility, potentially compromising bacterial persistence in the lung of patients.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00180-25","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T18:37:36Z","timestamp":1758911856000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Adaptation to cinnamaldehyde shapes\n                    <i>Pseudomonas aeruginosa<\/i>\n                    resistance to major antibiotics"],"prefix":"10.1128","volume":"207","author":[{"given":"Eline","family":"Dubois","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04asdee31","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249)","place":["Besan\u00e7on, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susie","family":"Gaillot","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04asdee31","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249)","place":["Besan\u00e7on, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"Valot","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04asdee31","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 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