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However, isolates with loss-of-function mutations in\n                      <jats:italic toggle=\"yes\">lasR<\/jats:italic>\n                      (LasR\u2013 strains) are commonly found in diverse settings, including infections where they are associated with worse clinical outcomes. In LasR\u2013 strains, the LasR-regulated transcription factor RhlR can also be stimulated by the activity of the two-component system PhoR-PhoB in low-inorganic phosphate (Pi) conditions. Here, we demonstrate a novel link between LasR and PhoB in which the absence of LasR increases PhoB activity at physiological Pi concentrations and increases the Pi concentration necessary for PhoB inhibition. PhoB activity was also less sensitive to repression by Pi in mutants lacking different QS regulators (RhlR and PqsR) and in mutants lacking genes required for QS-induced phenazine production, suggesting that decreased phenazine production is one reason for increased PhoB activity in LasR\u2013 strains. In addition, the CbrA-CbrB two-component system, which can be more active in LasR\u2013 strains, was necessary for increased PhoB activity in LasR\u2013 strains, and loss of the CbrA-CbrB-controlled translational repressor Crc was sufficient to activate PhoB in LasR+\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      . Phenazines and CbrA-CbrB affected PhoB activity independently. The \u2206\n                      <jats:italic toggle=\"yes\">lasR<\/jats:italic>\n                      mutant also had PhoB-dependent growth advantages in the Pi-deplete medium and increased virulence-associated gene expression at physiological Pi, in part through reactivation of QS. This work suggests PhoR-PhoB activity may contribute to the fitness and virulence of LasR\u2013\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      and subsequent clinical outcomes.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Loss-of-function mutations in the gene encoding the\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        quorum sensing (QS) regulator LasR occur frequently and are associated with worse clinical outcomes. We have found that LasR\u2013\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        have elevated PhoB activity at physiological concentrations of inorganic phosphate (Pi). PhoB activity promotes Pi acquisition as well as the expression of QS and virulence-associated genes. Previous work has shown that PhoB induces RhlR, another QS regulator, in a LasR\u2013 mutant in low-Pi conditions. Here, we demonstrate a novel relationship wherein LasR represses PhoB activity through the production of phenazines and Crc-mediated translational repression. This work suggests PhoB activity may contribute to the increased virulence of LasR\u2013\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jb.00189-24","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T09:05:01Z","timestamp":1747213501000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Loss of LasR function leads to decreased repression of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    PhoB activity at physiological phosphate concentrations"],"prefix":"10.1128","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4174-2268","authenticated-orcid":false,"given":"Amy","family":"Conaway","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dallas L.","family":"Mould","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Todorovic","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6366-2971","authenticated-orcid":true,"given":"Deborah A.","family":"Hogan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Clinical methods: the history, physical, and laboratory examinations","author":"Bansal VK","year":"1990","unstructured":"Bansal VK. 1990. 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